Anti-UBA1/7 Rabbit PolymAb® for WB, IF-P, IHC-P, ELISA - P22314 / P41226

Core Product Specifications and Parameters

Biological Background: UBA1/7 Function and Localization

• UBA1 (ubiquitin-like modifier-activating enzyme 1) is the primary E1 enzyme that initiates ubiquitin conjugation, targeting proteins for proteasomal degradation (PubMed:1447181, PubMed:1606621, PubMed:33108101). Related references: PMID:1447181 PMID:1606621 PMID:33108101
• It activates ubiquitin via ATP-dependent adenylation of its C-terminal glycine, forming a ubiquitin-E1 thioester intermediate essential for subsequent E2/E3 transfer (PubMed:1447181). Related references: PMID:1447181
• UBA1 plays a critical role in the DNA damage response by facilitating the formation of radiation-induced foci and recruiting repair proteins such as TP53BP1 and BRCA1 to damage sites (PubMed:22456334). Related references: PMID:22456334
• The protein localizes to multiple subcellular compartments, including the cytoplasm, nucleus, and mitochondria, consistent with its diverse functional roles.
• UBA1 is ubiquitously expressed in tissues, with detection confirmed in erythrocytes at the protein level.
• Post-translational modifications of UBA1 include acetylation and phosphorylation, and mutations in the gene are associated with neurodegenerative disorders and VEXAS syndrome.
• The UBA7 protein (ubiquitin-like modifier-activating enzyme 7) is a related E1 enzyme with distinct specificity for ISG15, but shares structural homology with UBA1, enabling cross-recognition by this antibody.

Experimental Guidance and Technical Tips

• The immunogen peptide (aa959-1058 of UBA1) lies within a region that shares significant homology with UBA7, explaining the dual-target specificity. Validate specificity using knockdown or knockout controls in your samples.
• In Western blotting (WB), expect predominant bands at ~118 kDa for UBA1 and ~112 kDa for UBA7, with possible variations depending on post-translational modifications or splice isoforms.
• For immunofluorescence (IF) and immunohistochemistry (IHC), note that UBA1 is distributed in the cytoplasm, nucleus, and mitochondria; optimize fixation and permeabilization conditions to preserve signal in these compartments.
• For ELISA applications, consider using recombinant UBA1 protein or cell lysates spiked with the target as standards, and perform cross-reactivity testing against UBA7 to confirm detection.
• As a recombinant rabbit monoclonal antibody (Rabbit PolymAb®), this reagent offers high lot-to-lot consistency; however, optimal working dilutions should be determined empirically for each assay and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop platform for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development from concept to clinic. The Anti-UBA1/7 Rabbit PolymAb® addresses key needs in ubiquitin pathway research and related disease area studies, providing a reliable high-consistency tool for multi-application detection. Leverage CamelBio's extensive supplier network and technical expertise to secure comprehensive raw-material solutions for your IVD projects.