Anti-UAP56/DDX39B Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13838

Core Product Specifications and Parameters

Biological Background: UAP56/DDX39B Function and Localization

• DDX39B (also known as UAP56, BAT1) is a 49 kDa spliceosome RNA helicase of the DEAD box family, sharing functional homology with the yeast Sub2 protein.
• It is a core component of the TREX (transcription/export) complex, coupling transcription, splicing, and nuclear export of mRNAs. DDX39B is recruited to spliced mRNAs in a cap-dependent manner and facilitates mRNA export via the NXF1/NXT1 pathway. Related references: PMID:15833825, PMID:15998806, PMID:17190602
• Within the TREX complex, the THOC2 subunit directly interacts with DDX39B and enhances its ATPase activity. Related references: PMID:33191911
• The RNA-binding adaptor SARNP/CIP29 associates with DDX39B and promotes its RNA binding, contributing to mRNA export. Related references: PMID:37578863
• DDX39B is required for the first ATP-dependent step in spliceosome assembly and interacts with U2 snRNP at the branchpoint. It possesses RNA-stimulated ATPase and ATP-dependent RNA unwinding activities, with a preference for single-stranded RNA substrates.
• In addition to its roles in mRNA processing and export, DDX39B contributes to transcription elongation and maintenance of genome stability.
• Subcellularly, DDX39B localizes to the nucleus, nuclear speckles, and cytoplasm, consistent with its shuttling role in RNA transport.
• The protein undergoes multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 319–428 near the C-terminus of human DDX39B; this region is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, a band at approximately 49 kDa is expected; consider using whole-cell lysates of human, mouse, or rat cell lines.
• In immunohistochemistry and immunofluorescence, anticipate nuclear and nuclear speckle staining, reflecting DDX39B's primary localization.
• The antibody is suitable for ELISA; consider using recombinant DDX39B or cell lysates as coating antigens and validate binding under optimized conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This UAP56/DDX39B polyclonal antibody is ideal for investigations into mRNA processing, splicing, and export, supporting research in oncology, infectious disease, and genetic disorders. Rely on CamelBio to source validated antibody pairs, bulk ancillary reagents, and rare-target raw materials for your IVD assay development and manufacturing needs.