Anti-UAP56/DDX39B Monoclonal Antibody for WB, IHC-P, ELISA - Q13838

Core Product Specifications and Parameters

Biological Background: UAP56/DDX39B Function and Localization

• Spliceosome RNA helicase DDX39B, also known as 56 kDa U2AF65-associated protein, ATP-dependent RNA helicase p47, DEAD box protein UAP56, and HLA-B-associated transcript 1 protein, is encoded by the DDX39B (BAT1, UAP56) gene.
• Component of the TREX complex, coupling mRNA transcription, processing, and nuclear export. Associates specifically with spliced mRNA and is recruited in a splicing- and cap-dependent manner near the 5′ end, functioning in mRNA export via the TAP/NXF1 pathway. Also involved in nuclear export of unspliced mRNA. Related references: PMID:15833825 PMID:15998806 PMID:17190602
• Required for the first ATP-dependent step in spliceosome assembly and U2 snRNP interaction with the branchpoint. Exhibits RNA-stimulated ATP binding/hydrolysis and ATP-dependent RNA unwinding activities; the helicase and ATPase activities are not influenced by U2AF2, though ALYREF/THOC4 has a reported stimulatory effect (PMID:23299939). Related references: PMID:23299939
• Associates with SARNP/CIP29, which facilitates RNA binding of DDX39B and likely plays a role in mRNA export. Related references: PMID:37578863
• Plays a role in intronless mRNA export; the TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.
• Subcellular localization is primarily nuclear, with presence in nuclear speckles, and also detectable in the cytoplasm.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), as indicated by proteomics identification.
• Belongs to the DEAD box helicase family; key motifs: ATP-binding, nucleotide-binding, RNA-binding. Involved in mRNA processing, mRNA splicing, mRNA transport, and genome stability.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 310-428), which is conserved in human, mouse, and rat; confirm sequence homology for other species if needed.
• For western blot, the predicted molecular weight is 49 kDa; validate with appropriate positive and negative controls in your sample system.
• For IHC-P, consider antigen retrieval and optimize primary antibody dilution based on tissue type; validate in tissues known to express UAP56/DDX39B.
• For ELISA, use recombinant protein as a standard and titrate the antibody concentration for optimal signal-to-noise ratio.
• The antibody is recommended for research use in mRNA processing, splicing, and nuclear export studies; performance may vary across applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting your workflow from concept to clinic. For studies on mRNA export, splicing, and transcriptional regulation, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare target raw materials to accelerate your diagnostic development.