Anti-UACA Rabbit Polyclonal Antibody for WB and ELISA - Q9BZF9

Core Product Specifications and Parameters

Biological Background: UACA Function and Localization

• UACA (Uveal autoantigen with coiled-coil domains and ankyrin repeats), also known as NUCLING, is a large protein with ankyrin repeats and coiled-coil domains, existing in multiple isoforms due to alternative splicing.
• It functions as a key regulator of apoptosis by controlling APAF1 expression, upregulating the apoptosome, downregulating LGALS3/galectin-3, and inactivating NF-kappa-B, thereby promoting programmed cell death under stress conditions.
• UACA also modulates isoactin dynamics to regulate cell morphology, growth, and motility; its interaction with ARF6 may influence cell shape and motility after injury, and it may participate in neurite formation.
• The protein localizes to the nucleus, cytoplasm, and cytoskeleton, reflecting its dynamic roles in both nuclear and cytoplasmic processes.
• It is highly expressed in skeletal muscle, heart, kidney, and pancreas, with additional presence in choroid, retina, epidermal melanocytes, eye muscles, and thyroid follicular cells.
• Post-translational modifications include acetylation and ubiquitination (isopeptide bond formation), and the protein features ANK repeats and coiled-coil domains essential for its interactions.
• With a molecular weight of approximately 162.5 kDa (163 kDa on SDS-PAGE), UACA is ubiquitously expressed but exhibits tissue-specific regulation.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (amino acids 1–90) of human UACA, which may contribute to recognition of both full-length and potential isoform variants; consider sequence alignment if working with highly homologous proteins.
• For Western blot, a band around 163 kDa is expected in relevant human, mouse, or rat samples; validate the antibody in your specific lysate and loading conditions, and consider using a high-molecular-weight marker.
• ELISA applications may require optimization of coating antigen and antibody dilution; the polyclonal nature can enhance sensitivity but also increase background—titration is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For UACA-related research, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. By integrating comprehensive supply capabilities with target-specific expertise, CamelBio supports your workflow from apoptosis studies to cytoskeleton research, ensuring reliable procurement and development support.