Anti-U2AF2/U2AF65 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P26368

Core Product Specifications and Parameters

Biological Background: U2AF2/U2AF65 Function and Localization

• U2AF2/U2AF65 is a splicing factor essential for pre-mRNA splicing and 3'-end processing by binding the polypyrimidine tract near the 3' splice site. Related references: PMID:17024186
• It may couple transcription to splicing by recruiting the PRP19 complex to the RNA polymerase II C-terminal domain. Related references: PMID:21536736
• The protein is localized in the nucleus, consistent with its role in mRNA processing.
• U2AF2 regulates alternative splicing events, such as inducing cardiac troponin-T (TNNT2) exon inclusion and repressing MAPT/Tau exon 10 splicing.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which may modulate its activity (UniProt P26368 keywords).
• It is required for mRNA export from the nucleus, even for intron-less transcripts.
• The UniProt record lists additional features like alternative splicing, intellectual disability-associated disease variants, and isopeptide bond formation, highlighting its functional complexity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 192-471 of human U2AF2. This region may include key functional domains; consider verifying sequence coverage against isoform-specific needs.
• For Western blot (WB), the predicted molecular weight is ~54 kDa; validate using appropriate positive control lysates from human or mouse cells/tissues.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect nuclear staining; use appropriate fixation and permeabilization protocols to preserve nuclear antigens.
• For immunoprecipitation (IP), the polyclonal nature may enhance capture efficiency, but confirm compatibility with downstream assays (e.g., western blot, mass spectrometry).
• ELISA suitability suggests the antibody can bind native or denatured protein; optimize coating conditions and antibody dilutions for quantitative assays.
• Cross-reactivity with mouse means it may be applied to mouse models, but always validate species-specific reactivity in the experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio supplies validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for pre-mRNA splicing research. This anti-U2AF2/U2AF65 antibody exemplifies our commitment to providing high-quality reagents for RNA processing studies, supporting your workflow from discovery to diagnostic development.