Anti-U2AF2/U2AF65 Monoclonal Antibody for WB, IHC-P, IP, ELISA - P26368

Core Product Specifications and Parameters

Biological Background: U2AF2/U2AF65 Function and Localization

• Splicing factor U2AF 65 kDa subunit (U2AF2/U2AF65) is a core component of the U2 auxiliary factor complex, essential for pre-mRNA splicing and 3'-end processing. Related references: PMID:17024186
• U2AF2 couples transcription to splicing by recruiting the PRP19 complex to the RNA polymerase II C-terminal domain (CTD) and pre-mRNA. Related references: PMID:21536736
• The protein regulates alternative splicing of specific transcripts; for example, it induces cardiac troponin-T (TNNT2) exon 5 inclusion by competing with MBNL1 and binding the polypyrimidine tract.
• U2AF2 localizes predominantly to the nucleus.
• It is also required for export of mRNA from the nucleus, even for intron-less genes.
• Represses the splicing of MAPT/Tau exon 10, contributing to tau isoform regulation.
• Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals. Related references: PMID:17024186
• Keywords from UniProt include phosphorylation, acetylation, hydroxylation, and ubiquitin conjugation, indicating dynamic post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 376-475 of human U2AF2, suggesting epitope specificity; verify specificity in the relevant sample system.
• When performing western blot (WB), the predicted protein weight is 54 kDa; consider using appropriate molecular weight markers and positive controls to confirm band identity.
• For immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized for the target tissue, and staining specificity validated with appropriate controls.
• The antibody is suitable for immunoprecipitation (IP) and ELISA; empirically determine optimal dilution factors and buffer conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated antibodies for splicing and mRNA processing studies. This U2AF2/U2AF65 monoclonal antibody exemplifies our catalog, and we offer bulk procurement, custom antibody development, and ancillary reagent sourcing to streamline your project from concept to clinic. For specialized or rare target needs, our sourcing network can help bridge supply gaps efficiently.