Anti-U2AF1 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q01081

Core Product Specifications and Parameters

Biological Background: U2AF1 (Splicing factor U2AF 35 kDa subunit) Function and Localization

• U2AF1 encodes the small 35 kDa subunit of the U2 auxiliary factor (U2AF), a heterodimeric splicing factor essential for 3' splice site recognition and U2 snRNP recruitment.
• In both constitutive and enhancer-dependent splicing, U2AF1 mediates critical protein-protein and protein-RNA interactions, acting as a bridge between the large U2AF2 subunit and enhancer-bound complexes.
• The U2AF1 protein contains two conserved zinc finger domains that facilitate RNA binding and spliceosomal assembly.
• U2AF1 localizes to the nucleus and is enriched in nuclear speckles, subnuclear domains where pre-mRNA splicing factors are concentrated.
• Post-translational modifications of U2AF1 include phosphorylation, acetylation, and methylation, which may regulate its activity and interactions during splicing.
• Alternative splicing of U2AF1 generates multiple isoforms, potentially contributing to tissue-specific or context-dependent splicing regulation.
• Mutations in U2AF1 are associated with disease variants, underscoring its importance in maintaining accurate pre-mRNA processing.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide spanning the N-terminal region (amino acids 1-100), which is likely to detect multiple U2AF1 isoforms if the epitope is conserved.
• Predicted protein weight is ~28 kDa; when performing Western blot, consider using a 10-15% SDS-PAGE gel for optimal separation.
• Although validated for WB, IHC-P, IP, and ELISA, optimal working dilutions and conditions should be empirically determined in each experimental system.
• Reported cross-reactivity with mouse and rat suggests the epitope is conserved, but independent validation in these species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, offering a one-stop sourcing solution for diagnostic manufacturers and research laboratories. This anti-U2AF1 polyclonal antibody is ideal for splicing research and can support assay development; CamelBio also supplies validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to facilitate your project from concept to clinical application.