Anti-U1A/SNRPA Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09012

Core Product Specifications and Parameters

Biological Background: U1A/SNRPA Function and Localization

• U1A (U1 small nuclear ribonucleoprotein A) is a component of the U1 snRNP, which plays a critical role in the recognition of the 5' splice site of pre-mRNA and the initial steps of spliceosome assembly. The U1 snRNP is the first to bind pre-mRNA, facilitating subsequent binding of U2 and the U4/U6/U5 tri-snRNP.
• SNRPA specifically binds stem-loop II of U1 snRNA, contributing to the stabilization of the U1 snRNP–pre-mRNA complex and accurate 5' splice site definition.
• In a snRNP-free form (SF-A), U1A may participate in the coupling of pre-mRNA splicing and polyadenylation, and it shows a binding preference for the 5'-UGCAC-3' motif on RNAs.
• The protein is localized exclusively in the nucleus, consistent with its role in pre-mRNA processing.
• U1A undergoes several post-translational modifications, including acetylation, phosphorylation, and methylation, which may regulate its activity and interactions.
• The molecular weight of the canonical isoform is approximately 31.3 kDa, and it belongs to the ribonucleoprotein family with characteristic RNA recognition motifs (RRMs).
• Key biological processes associated with U1A/SNRPA include mRNA splicing, mRNA processing, and spliceosome assembly, and it is a component of the U1 snRNP complex.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 31 kDa corresponding to U1A/SNRPA is expected. Validate detection using relevant nuclear extracts or whole-cell lysates from human, mouse, or rat samples.
• In immunohistochemistry (IHC-P), antigen retrieval methods may be necessary; optimize citrate or EDTA-based protocols, and include appropriate controls for nuclear staining.
• For immunofluorescence/immunocytochemistry (IF/ICC), ensure efficient nuclear permeabilization (e.g., Triton X-100 treatment) and consider co-staining with a spliceosome marker or DAPI to confirm nuclear localization.
• This antibody is suitable for ELISA; it can be used to detect recombinant or native U1A/SNRPA. Optimization of coating antigen concentration and antibody dilution is recommended.
• The immunogen spans the full-length protein (aa 1–282), making the antibody likely to recognize both native and denatured forms, but cross-reactivity with other U1 snRNP components should be ruled out by appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs and optimized polyclonal antibodies like this anti-U1A/SNRPA to support spliceosome and RNA processing research. We also supply bulk ancillary reagents and can source rare-target raw materials to accelerate your diagnostic development from concept to clinic.