Anti-Tyrosine Hydroxylase Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P07101

Core Product Specifications and Parameters

Biological Background: Tyrosine Hydroxylase Function and Localization

• Tyrosine hydroxylase (TH) is the rate-limiting enzyme in catecholamine biosynthesis, converting L-tyrosine to L-DOPA using tetrahydrobiopterin and molecular oxygen. This step is critical for the production of dopamine, noradrenaline, and adrenaline. Related references: PMID:15287903, PMID:1680128, PMID:17391063, PMID:24753243, PMID:34922205, PMID:8528210
• Alternative splicing produces multiple isoforms, some of which lack catalytic activity.
• TH is primarily expressed in the brain and adrenal glands, consistent with its role in neurotransmitter and hormone biosynthesis.
• Subcellularly, TH localizes to the cytoplasm, perinuclear region, nucleus, and is also found in cell projections such as axons and in synaptic vesicles.
• The protein undergoes phosphorylation and is associated with metal binding (iron), important for its enzymatic activity.
• Mutations in TH are linked to neurological disorders including DYT5b dystonia and Parkinson disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 411–528), which may allow detection of multiple TH isoforms; validate specificity in your target tissue or cell line.
• For Western blot, a band may appear between 45–59 kDa depending on isoform and phosphorylation state; consider loading sufficient protein and using appropriate positive controls (e.g., brain or adrenal lysates).
• IHC-P and IF/ICC may require antigen retrieval; include controls and optimize detection conditions for your sample type.
• ELISA applications can be used for quantitative detection; optimize coating concentration and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including this anti-Tyrosine Hydroxylase antibody for neuroscience and neurological disease studies. As a one-stop sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can facilitate sourcing of rare target raw materials to accelerate your assay development from concept to clinic.