Anti-Tyrosine Hydroxylase Monoclonal Antibody for WB, IHC, IF, ELISA - P07101

Core Product Specifications and Parameters

Biological Background: Tyrosine Hydroxylase Function and Localization

• Tyrosine Hydroxylase (TH, TYH) catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in catecholamine (dopamine, noradrenaline, adrenaline) biosynthesis.
  Related references: PMID:15287903 PMID:1680128 PMID:17391063 PMID:24753243 PMID:34922205 PMID:8528210
• Alternative splicing yields isoforms, some of which lack catalytic activity.
• TH is predominantly expressed in the brain and adrenal glands.
• The enzyme localizes to the cytoplasm, perinuclear region, nucleus, neuronal axons, and synaptic vesicles.
• TH functions as a phosphoprotein, binds iron, and is associated with dystonia (DYT14, DYT5b) and Parkinson’s disease.
• TH can also hydroxylate phenylalanine and tryptophan with lower efficiency.
• It is involved in the positive regulation of retinal hyaloid vessel regression during postnatal development.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 411-528) of human TH, suggesting the antibody may recognize all full-length isoforms.
• For Western blotting, a band around 59 kDa is expected; consider using brain or adrenal gland lysates as positive controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate fixation and permeabilization conditions, as TH localizes to both cytoplasmic and nuclear compartments.
• Cross-reactivity with mouse and rat is reported; however, verify species-specific reactivity in your experimental system.
• For ELISA applications, consider pairing with a detection antibody recognizing a different epitope to avoid competition.
• This recombinant rabbit monoclonal antibody offers batch-to-batch consistency suitable for reproducible assays.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, from validated antibody pairs to recombinant antigens. This anti-Tyrosine Hydroxylase monoclonal antibody is part of our offering for neurological disease research, supporting catecholamine and Parkinson’s studies. Beyond individual products, we streamline procurement of bulk ancillary reagents and rare-target antibodies, serving as a single-source bridge from discovery to clinical application.