Anti-Tyrosine Hydroxylase Monoclonal Antibody for WB, IF, IHC-P, and ELISA - P07101

Core Product Specifications and Parameters

Biological Background: Tyrosine Hydroxylase (TH) Function and Localization

• Tyrosine hydroxylase (TH) is a monooxygenase that catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in catecholamine biosynthesis (dopamine, noradrenaline, adrenaline), utilizing tetrahydrobiopterin and molecular oxygen as cofactors. Related references: PMID:15287903, PMID:1680128, PMID:17391063, PMID:24753243, PMID:34922205, PMID:8528210
• TH belongs to the aromatic amino acid hydroxylase family and requires iron as a metal cofactor for enzymatic activity.
• Alternative splicing of the TH gene produces multiple isoforms; some of these lack catalytic activity.
• TH is predominantly expressed in the brain and adrenal glands, consistent with its role in neurotransmitter production.
• Subcellularly, TH localizes to the cytoplasm, perinuclear region, nucleus, axon, and synaptic vesicles.
• Key post-translational modifications, especially phosphorylation, regulate TH activity and stability.
• Mutations in TH are associated with neurological disorders, including DYT5b dystonia and Parkinson's disease.
• TH can also hydroxylate phenylalanine and tryptophan with lower specificity.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band between 45-59 kDa; validate detection in your sample system and consider the potential presence of isoforms.
• In immunofluorescence applications, optimize fixation and permeabilization, and confirm staining in TH-positive tissues (e.g., brain, adrenal gland sections).
• The peptide immunogen suggests high epitope specificity; however, confirm cross-reactivity in your species of interest as the antibody is reported to react with human, mouse, and rat.
• For ELISA, pair with an appropriate capture/detection antibody and validate using recombinant TH or tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated monoclonal antibodies like this anti-Tyrosine Hydroxylase antibody, ideal for neuroscience and catecholamine biosynthesis research. Beyond single reagents, we supply optimized antibody pairs, bulk ancillary materials, and rare-target sourcing to accelerate your diagnostic development from concept to clinic.