Anti-Tyrosinase Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P14679

Core Product Specifications and Parameters

Biological Background: Tyrosinase Function and Localization

• Tyrosinase is a copper-containing oxidase that catalyzes the initial and rate-limiting step in melanin production: the hydroxylation of tyrosine to DOPA, and subsequently the oxidation of DOPA to DOPA-quinone. Related references:
  PMID:28661582
• The enzyme is also known as Monophenol monooxygenase, Tumor rejection antigen AB, and is associated with pigmentation disorders such as oculocutaneous albinism type 1 (OCA1) and Waardenburg syndrome.
• Tyrosinase localizes to the melanosome membrane and melanosome, where it functions in pigment production.
• Post-translational modifications include glycosylation; the protein is a glycoprotein.
• It is a membrane-bound protein with a single transmembrane helix.
• Tyrosinase is classified as a tumor antigen and may be involved in immune recognition in melanoma.
• Alternative splicing gives rise to multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen (aa 20-340) covers a large portion of the protein, including the core catalytic domain; consider using this antibody for detecting both precursor and mature forms of Tyrosinase.
• For Western blot, human melanoma cell lines (e.g., SK-MEL-28) or melanocyte-rich tissues may serve as positive controls; the predicted molecular weight is approximately 60 kDa, though glycosylation may cause shifts.
• In IHC-P, note that melanin pigment in melanocytes can cause background; appropriate blocking and detection methods should be employed to distinguish specific signal.
• This antibody is reactive with human and rat, allowing cross-species studies in rodent pigmentation models.
• For ELISA, consider using the recombinant fusion protein used as immunogen as a coating antigen or a standard for quantification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, streamlining access to high-quality antibodies like this anti-Tyrosinase polyclonal antibody for melanin biosynthesis and melanoma research. Beyond single antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and customized rare-target sourcing to accelerate your diagnostic development pipeline.