Anti-TYROBP Polyclonal Antibody for WB, ELISA - O43914

Core Product Specifications and Parameters

Biological Background: TYROBP Function and Localization

• TYROBP, also known as DAP12 or KARAP, is an adapter protein that associates with various activating receptors on immune cells, mediating intracellular signaling and cell activation upon ligand binding. Related references: PMID:10604985, PMID:9490415, PMID:9655483
• It contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain; tyrosine phosphorylation of this ITAM following receptor engagement recruits and activates downstream tyrosine kinases, leading to cell activation. Related references: PMID:9490415
• TYROBP also exhibits an inhibitory role in certain contexts, such as negative regulation of B cell proliferation. Related references: PMID:21727189
• It associates with receptors of the CD300 and CD200R families, as well as triggering receptors expressed on myeloid cells (TREMs) like TREM1 and TREM2, to regulate myeloid cell activation, dendritic cell maturation, and macrophage fusion. Related references: PMID:15557162, PMID:16920917, PMID:17928527, PMID:10799849, PMID:11602640, PMID:18957693
• In natural killer (NK) cells, TYROBP couples with receptors like KIR2DS2 and the KLRD1/KLRC2 heterodimer to mediate NK cell activation, and it enhances trafficking and surface stability of some KIR receptors. Related references: PMID:23715743, PMID:9490415, PMID:9655483
• TYROBP is localized to the cell membrane and is a transmembrane protein with a signal peptide.
• It is predominantly expressed in hematopoietic tissues and cells, including monocytes, peripheral blood leukocytes, spleen, and bone marrow, with lower expression in brain regions such as basal ganglia. Keywords include: Immunity, Phosphoprotein, Metal-binding, Calcium, Disulfide bond.
• TYROBP undergoes alternative splicing and has a molecular weight of approximately 12 kDa.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was generated against a synthetic peptide corresponding to amino acids 1-113 of human TYROBP (NP_003323.1), covering nearly the entire protein sequence.
• In Western blot (WB), a band at approximately 12 kDa is anticipated; researchers should validate specificity using appropriate positive controls such as human monocyte or spleen lysates.
• The antibody is reported to cross-react with mouse and rat TYROBP; however, optimal working dilutions and conditions should be determined empirically in the relevant sample system.
• For ELISA applications, consider pairing with a matched detection antibody to develop a sandwich assay; the polyclonal nature may provide signal amplification.
• As TYROBP is expressed in hematopoietic cells and immune tissues, performations in these cell types or tissues are recommended for initial testing.
• This product is shipped on ice bags; upon receipt, store at recommended temperatures and minimize freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For TYROBP and other immune signaling targets, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials to accelerate your assay development and manufacturing workflows.