Anti-Tyro3 Monoclonal Antibody for WB and ELISA - Q06418

Core Product Specifications and Parameters

Biological Background: Tyro3 Function and Localization

• Tyro3 (also known as BYK, DTK, RSE, SKY, TIF) is a receptor tyrosine kinase that transduces signals from the extracellular matrix upon binding ligands such as GAS6 and TULP1.
• The receptor regulates physiological processes including cell survival, migration, and differentiation through activation of the PI3K–AKT pathway and NF-kappa-B nuclear translocation.
• Tyro3 signaling inhibits Toll-like receptor (TLR)-mediated innate immune responses by STAT1-dependent induction of SOCS1 and SOCS3.
• It is a single-pass transmembrane protein localized to the cell membrane, containing immunoglobulin-like domains, fibronectin type III repeats, and an intracellular tyrosine kinase domain.
• Post-translational modifications include phosphorylation on tyrosine residues, N-linked glycosylation, and disulfide bond formation.
• In humans, Tyro3 is most abundantly expressed in the brain, with lower levels in other tissues.
• Tyro3 serves as a host cell receptor for enveloped viruses including Lassa virus, lymphocytic choriomeningitis virus, and Ebolavirus, likely via GAS6-mediated binding to phosphatidylserine on the viral envelope.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an intracellular C-terminal region (aa 791–890); a band of ~97 kDa is expected in WB with human, mouse, or rat brain lysates.
• For ELISA, consider coating with the immunogen peptide or recombinant Tyro3 extracellular domain; always include appropriate controls.
• Enrich membrane fractions from cell or tissue lysates to improve detection of this transmembrane receptor.
• Cross-reactivity with mouse and rat is reported; nevertheless, verify specificity in your sample system due to potential sequence variations.
• The antibody may recognize both total and phosphorylated Tyro3; use phospho-specific tools to distinguish activation states.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio connects diagnostic developers, laboratories, and research institutes with high-quality reagents like this Tyro3 monoclonal antibody. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets such as receptor tyrosine kinases involved in viral pathogenesis, supporting your assay development from concept to clinic.