Anti-TYK2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P29597

Core Product Specifications and Parameters

Biological Background: TYK2 Function and Localization

• Protein Identity: TYK2 (also known as JTK1, IMD35) is a non-receptor tyrosine-protein kinase encoded by the TYK2 gene (GeneID: 7297) and belongs to the Janus kinase (JAK) family.
• Core Signaling Function: TYK2 plays a central role in cytokine and interferon signaling, regulating cell growth, development, migration, innate and adaptive immunity.
  Related references: PMID:10542297, PMID:10995743, PMID:7657660
• Receptor Complex Assembly: TYK2 associates with heterodimeric cytokine receptor complexes. One chain typically binds TYK2 (e.g., IFNAR1, IL12RB1, IL10RB, IL13RA1), while the second chain binds JAK1 or JAK2.
  Related references: PMID:10542297, PMID:25762719, PMID:7526154
• STAT Activation: Upon cytokine binding, TYK2 phosphorylates receptor chains to create docking sites for STAT transcription factors, and directly phosphorylates STAT1, STAT3, STAT4, or STAT6, leading to their dimerization, nuclear translocation, and target gene expression.
  Related references: PMID:10542297, PMID:7638186, PMID:7657660, PMID:7526154, PMID:25762719
• Negative Regulation of STAT3: TYK2 negatively regulates STAT3 activity by promoting phosphorylation at a distinct tyrosine residue, independent of the activating phosphorylation site.
  Related references: PMID:29162862
• Oncostatin-M Signaling: TYK2 mediates oncostatin-M signaling through both type I (LIFR/IL6ST) and type II (OSMR/IL6ST) receptor complexes.
  Related references: PMID:9188471
• Tissue Expression: TYK2 is ubiquitously expressed across lymphoid and non-lymphoid cell lines.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein corresponding to amino acids 1102-1182, representing a C-terminal region of human TYK2. For detection, consider validating specificity via peptide blocking or siRNA knockdown in relevant cell lines.
• Western blotting (WB): A band near 134 kDa is expected for endogenous TYK2. Use reducing conditions and appropriate loading controls; pre-test the antibody at dilutions from 1:500–1:2000 based on your sample system.
• Immunohistochemistry (IHC-P): Antigen retrieval and endogenous peroxidase blocking recommended. Validate staining in known positive tissues (e.g., lymphoid organs) and include isotype controls.
• Immunofluorescence (IF/ICC): Include permeabilization step for intracellular detection. Co-stain with organelle markers to confirm cytoplasmic localization.
• ELISA: Can be used for capture/detection in sandwich ELISA setups. Pair with monoclonal antibodies targeting distinct epitopes for optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing bridge for diagnostic manufacturers and research laboratories, offering validated raw materials including antibody pairs, bulk polyclonal antibodies, and ancillary reagents for immunology and signal transduction research. Our TYK2 rabbit polyclonal antibody is part of a broader portfolio for cytokine signaling pathways, and we can assist with procurement of rare-target antibodies and customized production services to accelerate your assay development.