Anti-TXNL4A Rabbit Polyclonal Antibody for WB and ELISA - P83876

Core Product Specifications and Parameters

Biological Background: TXNL4A Function and Localization

• TXNL4A, also known as Thioredoxin-like protein 4A, DIM1 protein homolog, or U5-15kD, is an evolutionarily conserved component of the spliceosome.
• It is a core subunit of the U5 small nuclear ribonucleoprotein (snRNP) and the U4/U6-U5 tri-snRNP complex, essential for pre-mRNA splicing.
• Functions in the assembly of the spliceosome and is present in the precatalytic spliceosome (B complex), facilitating the transition to catalytically active states.
• Localizes exclusively to the nucleus, consistent with its role in mRNA processing.
• The protein contains a thioredoxin-like domain; structural studies (3D-structure) have informed its interactions within the spliceosomal machinery.
• Post-translational modifications include phosphorylation, and it has been identified by proteomics in various cellular contexts.
• Mutations in TXNL4A are linked to autosomal recessive non-syndromic deafness, highlighting its tissue-specific importance in the inner ear.
• Listed keywords include Cell cycle, Cell division, Mitosis, mRNA processing, mRNA splicing, and Spliceosome, reflecting its participation in fundamental cellular processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human TXNL4A (amino acids 1-142), which is highly conserved; cross-reactivity with mouse is confirmed, but for other species, validate by sequence alignment.
• For Western blot, the predicted molecular weight is approximately 17 kDa; use appropriate gel percentage and loading controls. Optimize primary antibody dilution and exposure time for your specific samples.
• For ELISA, ensure the recombinant protein or peptide coating matches the immunogen region; consider using a blocking buffer that minimizes background from rabbit polyclonal sera.
• Since this is a polyclonal antibody, lot-to-lot variability may occur; verify performance with positive and negative controls in your assay.
• In immunofluorescence or immunohistochemistry, note that nuclear localization may require permeabilization and antigen retrieval optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers one-stop access to IVD raw materials, including validated antibody pairs and bulk ancillary reagents. This anti-TXNL4A polyclonal antibody is suitable for spliceosome and mRNA processing studies, and we can support your development with additional rare target raw-material sourcing and custom production. Whether you need monoclonal or polyclonal antibodies, or technical consulting from concept to clinic, CamelBio is your reliable partner.