Anti-TXNIP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H3M7

Core Product Specifications and Parameters

Biological Background: TXNIP Function and Localization

• TXNIP (Thioredoxin-interacting protein), also known as VDUP1 or TBP-2, is a key mediator of oxidative stress by inhibiting thioredoxin activity or limiting its bioavailability (PubMed:17603038).
  Related references: PMID:17603038
• It functions as a transcriptional repressor, potentially bridging transcription factors and corepressor complexes, and overexpression induces G0/G1 cell cycle arrest (PubMed:12821938).
  Related references: PMID:12821938
• TXNIP acts as a tumor suppressor by inhibiting cell growth, as demonstrated in various cancer models (PubMed:18541147).
  Related references: PMID:18541147
• The protein inhibits the proteasomal degradation of DDIT4, thereby contributing to mTORC1 inhibition (PubMed:21460850).
  Related references: PMID:21460850
• TXNIP interacts with COPS5 to restore CDKN1B stability and prevent its nuclear-to-cytoplasmic translocation, further modulating cell cycle progression.
• Predominantly localizes to the cytoplasm and nucleus, and is subject to post-translational modifications including phosphorylation and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-166), which may be suitable for detecting full-length TXNIP in WB.
• For WB, consider using positive controls such as HeLa or HEK293 cell lysates, known to express TXNIP.
• In IF/ICC, expect both cytoplasmic and nuclear staining due to the protein’s dual subcellular localization.
• Validate antibody specificity in your sample system by siRNA‑mediated knockdown or antigen competition as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, including validated antibodies and ancillary reagents. Our TXNIP antibody supports research into oxidative stress, cell cycle regulation, and cancer biology. We also offer custom reagent development and rare target sourcing to accelerate your diagnostic development from concept to clinic.