Anti-TXNDC9 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O14530

Core Product Specifications and Parameters

Biological Background: TXNDC9 Function and Localization

• TXNDC9 (Thioredoxin domain-containing protein 9), also known as APACD (ATP-binding protein associated with cell differentiation) and Protein 1-4, is encoded by the TXNDC9 gene in humans.
• It acts as a negative regulator of the chaperonin TCP1 complex (CCT/TRiC) by significantly reducing its ATPase activity, thereby impairing the folding of key cytoskeletal proteins such as actin and tubulin.
• Subcellular localization studies indicate TXNDC9 is present in the cytoplasm, nucleus, and associates with the cytoskeleton, centrosome, and midbody during cell division.
• The protein is subject to phosphorylation and alternative splicing, as identified through proteomics approaches.
• A potential role in cell differentiation is suggested by its alias APACD, though detailed molecular pathways remain to be fully elucidated.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, the predicted molecular weight is approximately 27 kDa; validate band specificity using appropriate controls, such as knockdown or knockout cell lysates.
• In immunohistochemistry (paraffin-embedded sections, IHC-P), antigen retrieval may be required; test staining in control tissues known to express TXNDC9.
• For ELISA applications, the recombinant immunogen (amino acids 1-110) can be used as a positive control; optimal coating and antibody dilution should be determined experimentally.
• Given the reported cross-reactivity with human, mouse, and rat, users should confirm reactivity in their specific sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between discovery and diagnostics with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents. Our TXNDC9 rabbit polyclonal antibody supports research in protein folding, cytoskeletal dynamics, and cell cycle regulation. Trust CamelBio for consistent supply, technical expertise, and custom sourcing to accelerate your assay development from concept to clinic.