Anti-TXNDC5 Polyclonal Antibody for WB - Q8NBS9

Core Product Specifications and Parameters

Biological Background: TXNDC5 Function and Localization

• TXNDC5 (Thioredoxin domain-containing protein 5), also known as ERp46 or endoplasmic reticulum resident protein 46, is a protein disulfide isomerase localized to the endoplasmic reticulum lumen.
• It catalyzes the formation of disulfide bonds in nascent proteins, facilitating proper protein folding and maintaining ER homeostasis.
• The protein possesses a thioredoxin-like redox-active center that enables oxidoreductase activity, including the reduction of insulin disulfide bonds in vitro.
• Alternative splicing generates multiple isoforms; a signal peptide directs the nascent polypeptide to the ER.
• Disulfide bond formation is a key post-translational modification critical for the enzyme's catalytic function and structural stability.
• Proteomics studies confirm its exclusive ER luminal localization, consistent with its role in early secretory pathway processing.
• It belongs to the protein disulfide isomerase family and is implicated in the unfolded protein response and ER stress signaling.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1-324 of human TXNDC5 (NP_001139021.1), covering the N-terminal region; the antibody is expected to recognize all isoforms containing this segment.
• For western blot, the predicted molecular weight is approximately 48 kDa; validate band identity in your specific sample matrix.
• The antibody shows cross-reactivity with mouse and rat TXNDC5, enabling its use in rodent models of ER function.
• Optimization of blocking conditions and antibody dilution is recommended for IHC-P and IF/ICC applications, as epitope accessibility may vary with fixation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, offering a comprehensive portfolio of validated antibodies, bulk reagents, and ancillary products to accelerate diagnostic development. This TXNDC5 polyclonal antibody supports research into ER stress, protein folding, and related pathologies. Whether you need matched antibody pairs, rare target sourcing, or technical consultation, CamelBio bridges the gap from concept to clinical application.