Anti-TXNDC12 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95881

Core Product Specifications and Parameters

Biological Background: TXNDC12 Function and Localization

• TXNDC12 (Thioredoxin domain-containing protein 12), also designated ERp18/ERp19 or hTLP19, is a member of the protein disulfide isomerase (PDI) family localized to the endoplasmic reticulum (ER).
• It functions as a thiol-disulfide oxidoreductase, catalyzing disulfide bond formation, reduction, and isomerization in nascent polypeptides to ensure correct oxidative protein folding in the ER.
• The catalytic activity of TXNDC12 relies on a redox-active CXXC motif within its thioredoxin-like domain, which cycles between reduced and oxidized states to exchange disulfides with substrates.
• Subcellular fractionation and immunofluorescence studies confirm that TXNDC12 resides in the ER lumen, consistent with its role in co- and post-translational protein maturation.
• TXNDC12 is broadly expressed across human tissues, reflecting its housekeeping function in maintaining ER proteostasis.
• Alternative names include AG1, AGR1, ERP16, ERP18, ERP19, TLP19, hAG-1, PDIA16, and hTLP19, reflecting historical discovery in various contexts.
• Sequence analysis reveals a signal peptide for ER targeting, and direct protein sequencing corroborates the full-length protein.
• The protein has a molecular weight of approximately 19 kDa (calculated mass ~19.2 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 93-172 of human TXNDC12, a C-terminal region that is highly conserved across human, mouse, and rat, explaining the observed cross-reactivity with these species.
• For Western blotting (WB), a predominant band at approximately 19 kDa is expected under reducing conditions; treat samples with reducing agents to disrupt disulfide bonds for optimal resolution.
• When performing immunohistochemistry (IHC-P) or immunofluorescence (IF/ICC), consider heat-induced antigen retrieval and validate staining specificity with tissue or cell lines known to express TXNDC12, such as secretory tissues.
• For ELISA applications, coat the recombinant protein or peptide immunogen as a positive binding control to confirm antibody reactivity.
• As a polyclonal antibody, lot-to-lot variability can occur; always include appropriate positive and negative controls and consider batch testing for critical experiments.
• It is advisable to titrate the antibody and establish optimal working concentrations for each assay system individually.

CamelBio: Your One-Stop Sourcing Bridge

As an IVD raw material specialist, CamelBio offers comprehensive access to validated antibodies, antibody pairs, and ancillary reagents for immunoassay development. This anti-TXNDC12 polyclonal antibody supports research into ER protein folding, redox regulation, and protein disulfide isomerase-related diseases. Whether you require bulk supply, custom production, or technical consultation from concept to clinic, CamelBio is your reliable sourcing bridge.