Anti-TXLNA Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P40222

Core Product Specifications and Parameters

Biological Background: Alpha-taxilin Function and Localization

• Alpha-taxilin is a protein encoded by the TXLNA gene (aliases: IL14, TXLN) in humans.
• It is predicted to be involved in intracellular vesicle trafficking, particularly in neuroendocrine cells, where it may participate in calcium-dependent exocytosis.
• Expression is ubiquitous, but markedly higher in heart, kidney, liver, and pancreas.
• The protein contains coiled-coil domains, suggesting a role in mediating protein–protein interactions.
• Alpha-taxilin is annotated as a phosphoprotein, implying potential regulation by phosphorylation events.
• The calculated molecular weight is approximately 61.9 kDa, consistent with an observed band around 62 kDa in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–160); therefore, the antibody is expected to recognize epitopes within this segment. Consider this when designing epitope mapping or blocking experiments.
• For Western blot, expect a band near 62 kDa. Use lysates from high-expression tissues (e.g., heart, kidney, liver) as positive controls and include appropriate molecular weight markers.
• In immunofluorescence/ICC, permeabilization is required to detect intracellular alpha-taxilin. Optimize permeabilization conditions and validate in cell lines with known expression.
• For ELISA, titrate both the coating antigen (full-length or N-terminal recombinant TXLNA) and antibody dilution to determine optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, research laboratories, and IVD developers with validated raw materials, including this polyclonal antibody targeting alpha-taxilin for trafficking and exocytosis studies. Beyond single antibodies, we supply matched antibody pairs, bulk ancillary reagents, and customized sourcing for rare or emerging targets—streamlining your pathway from concept to clinical application.