Anti-TWIST1 Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q15672

Core Product Specifications and Parameters

Biological Background: TWIST1 Function and Localization

• TWIST1, also known as H-twist, is a class A basic helix-loop-helix (bHLH) transcription factor encoded by the TWIST1 gene.
• It functions as a transcriptional regulator, inhibiting myogenesis by sequestering E proteins, blocking MEF2 transactivation, and disrupting MYOD1 DNA-binding through direct interaction.
• TWIST1 represses expression of pro-inflammatory cytokines such as TNFA and IL1B, and regulates cranial suture patterning and fusion.
• Depending on its dimerization partner, TWIST1 can either activate or repress target genes; for example, homodimers induce FGFR2 and POSTN, while heterodimers repress these and induce THBS1.
• Heterodimer formation with E proteins is required for osteoblast differentiation, highlighting its role in bone development.
• In the nucleus, TWIST1 also represses the circadian transcriptional activator complex NPAS2-BMAL1, linking it to biological rhythm regulation.
• TWIST1 is specifically expressed in a subset of mesodermal cells during development, consistent with its role in differentiation and morphogenesis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 50–150 of human TWIST1; this region is suitable for generating antibodies that recognize the native protein in WB, IF/ICC, IF-P, and ELISA applications.
• For western blot, a band corresponding to TWIST1 at approximately 21 kDa is expected; use appropriate positive controls such as human or mouse cell lysates expressing TWIST1.
• In immunofluorescence and immunocytochemistry, confirm nuclear localization of TWIST1; permeabilization and blocking conditions may need optimization for your specific cell type.
• ELISA performance may depend on plate coating and detection conditions; consider testing with recombinant TWIST1 protein or cell extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions for diagnostic manufacturers and research groups. This TWIST1 monoclonal antibody supports investigations into transcriptional regulation, developmental biology, and disease mechanisms. Beyond antibodies, we supply validated antibody pairs, bulk ancillary reagents, and can assist with sourcing rare-target raw materials to accelerate your assay development from concept to clinic.