Anti-Twist Polyclonal Antibody for WB, IHC-P, ELISA - Q15672

Core Product Specifications and Parameters

Biological Background: Twist Function and Localization

• TWIST1 (Twist-related protein 1) is a class A basic helix-loop-helix (bHLH) transcription factor, also known as bHLHa38 or H-twist.
• Functions as a transcriptional regulator, playing a critical role in craniofacial development by regulating cranial suture patterning and fusion.
• Inhibits myogenesis through multiple mechanisms, including sequestration of E proteins, inhibition of MEF2 transactivation, and physical interaction with MYOD1 to block its DNA binding.
• Represses expression of pro-inflammatory cytokines such as TNFA and IL1B, and also represses the activity of the circadian NPAS2-BMAL1 transcriptional activator complex (by similarity).
• Dimerization status modulates gene expression: homodimers induce FGFR2 and POSTN, while heterodimers with E proteins repress these genes and induce THBS1; heterodimerization is required for osteoblast differentiation.
• Localized to the nucleus and predominantly expressed in a subset of mesodermal cells.
• Keywords: activator, biological rhythms, craniosynostosis, developmental protein, differentiation, disease variant, DNA-binding, myogenesis, repressor, transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-100 of human Twist (NP_000465.1), an N-terminal region; consider its suitability for detecting total Twist protein levels in your system.
• For Western blot (WB), the predicted molecular weight is 21 kDa; use a 12-15% SDS-PAGE gel and a low-fluorescence PVDF membrane for efficient transfer, and validate specificity using appropriate positive/negative controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and validate the antibody in mesoderm-derived tissue samples, given Twist expression in a subset of mesodermal cells.
• In ELISA applications, titrate the antibody according to your assay format and consider pairing with a capture antibody if developing a sandwich ELISA.
• The antibody shows cross-reactivity with mouse and rat; we recommend verifying species reactivity in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, delivering a comprehensive portfolio of antibodies, antigens, and ancillary reagents from concept to clinic. This Twist polyclonal antibody exemplifies our capability to supply high-quality research tools for transcription factor studies. We support diagnostic manufacturers and laboratories with validated antibody pairs, bulk orders, and custom sourcing for rare or specialized targets, ensuring a seamless one-stop procurement experience.