Anti-TWF2 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q6IBS0

Core Product Specifications and Parameters

Biological Background: TWF2 Function and Localization

• Twinfilin-2 (also known as A6-related protein, PTK9L, MSTP011) is an actin-binding protein encoded by the TWF2 gene in humans.
• It inhibits actin polymerization by sequestering G-actin and capping the barbed ends of actin filaments, thereby regulating cytoskeletal dynamics and cell motility.
• Plays an important role in clathrin-mediated endocytosis and the distribution of endocytic organelles.
• Involved in regulating the mature length of the middle and short rows of stereocilia in hair cells.
• Localizes to the cytoplasm, with enrichment in the perinuclear region and association with the cytoskeleton. Also found at cell projections, specifically stereocilia.
• Ubiquitously expressed at the protein level.
• Contains actin-binding repeats and is subject to post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole cell lysates from human, mouse, or rat cells; the expected band is around 40 kDa. Optimize blocking and antibody dilution conditions for your specific sample system.
• In immunoprecipitation experiments, validate elution and wash conditions to ensure efficient capture of the target protein.
• For ELISA applications, the antibody may be used for direct antigen coating or in a sandwich format; we recommend titration and buffer optimization.
• As the immunogen covers amino acids 1-349, the antibody likely recognizes the full-length protein; however, always verify specificity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories as a comprehensive IVD raw-material partner. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For TWF2-related cytoskeleton and endocytosis research, our supply chain covers everything from recombinant proteins to matched detection systems. Let us help you streamline your raw-material procurement and accelerate your assay development.