Anti-TWF2 Polyclonal Antibody for WB, ELISA - Q6IBS0

Core Product Specifications and Parameters

Biological Background: TWF2 Function and Localization

• TWF2 encodes twinfilin-2, also known as A6-related protein (hA6RP) or PTK9L, a conserved actin-binding protein.
• Functions as an actin-depolymerizing factor by sequestering G-actin and capping barbed ends of filaments, thereby inhibiting actin polymerization and regulating cytoskeletal dynamics.
• Essential for clathrin-mediated endocytosis by controlling actin turnover at endocytic sites, influencing the distribution of endocytic organelles.
• Localizes to the cytoplasm, cytoskeleton, perinuclear region, and cell projections such as stereocilia, where it may regulate mature length of stereocilia rows.
• Ubiquitously expressed in human tissues at the protein level.
• Post-translationally modified by acetylation and phosphorylation, with structural studies revealing a conserved twinfilin domain architecture.

Experimental Guidance and Technical Tips

• The immunogen used is full-length recombinant human TWF2 (aa 1-349), so the polyclonal antibody likely recognizes multiple linear and conformational epitopes, suitable for detecting endogenous protein in both reducing and native conditions.
• For Western blot, a band around 40 kDa is expected; consider using whole cell lysates from human or mouse cell lines (e.g., HeLa, MCF-7, NIH/3T3) and validate with appropriate controls.
• In ELISA, the antibody can be used as a capture or detection reagent; titrate using recombinant TWF2 protein and validate with biological samples.
• For immunohistochemistry or immunofluorescence, cross-reactivity with mouse suggests potential for use in mouse tissues, but optimization is required due to the polyclonal nature.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as your comprehensive partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our supply network supports rare target sourcing for actin cytoskeleton and endocytosis research, ensuring seamless integration from concept to diagnostic development. Let CamelBio bridge your procurement needs with precision and reliability.