Anti-TWF1 Polyclonal Antibody for WB, ELISA - Q12792

Core Product Specifications and Parameters

Biological Background: Twinfilin-1 Function and Localization

• Twinfilin-1 (TWF1), also known as Protein A6 or Protein tyrosine kinase 9 (PTK9), is an actin-binding protein encoded by the TWF1 gene in humans.
• It inhibits actin polymerization by sequestering globular actin (G-actin) and capping the barbed ends of actin filaments, thereby regulating cell motility and morphological processes.
• TWF1 plays a significant role in clathrin-mediated endocytosis and the distribution of endocytic organelles.
• The protein localizes predominantly to the cytoplasm and is associated with the cytoskeleton.
• Tissue expression is highest in colon, testis, ovary, prostate, and lung, with lower levels in brain, bladder, and heart; it is absent in the liver.
• TWF1 undergoes alternative splicing and post-translational modifications including phosphorylation and acetylation, and is implicated in disease variants.

Experimental Guidance and Technical Tips

• Verify antibody specificity for your application; the recommended starting dilution for Western blot may need optimization in your experimental system.
• The immunogen corresponds to amino acids 101-350 of human TWF1; expect detection of a band at approximately 40 kDa. Consider including positive controls such as lysates from colon or testis tissue where TWF1 is highly expressed.
• This antibody has been shown to cross-react with mouse TWF1, making it potentially suitable for rodent model studies; however, validation in the specific mouse strain or tissue is advised.
• For ELISA, use a suitable coating antigen and validate linearity and specificity with your own standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibodies for targets like TWF1 involved in actin dynamics. Our portfolio includes polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents, supporting assay development from concept to clinic. For customized bulk orders or rare target sourcing, our team offers tailored solutions to meet your workflow needs.