Anti-TWEAKR/Fn14/CD266 Rabbit Monoclonal Antibody for WB, ELISA - Q9NP84

Core Product Specifications and Parameters

Biological Background: TWEAKR/Fn14/CD266 Function and Localization

• Tumor necrosis factor receptor superfamily member 12A (TNFRSF12A), also known as TWEAKR, Fn14, or CD266, is a transmembrane receptor encoded by the TNFRSF12A gene.
• Functions as the receptor for TNFSF12/TWEAK, acting as a weak inducer of apoptosis in certain cell types while promoting angiogenesis and endothelial cell proliferation.
• May modulate cellular adhesion to extracellular matrix proteins, implicating involvement in cell-matrix interactions.
• Localized to the membrane, with a signal peptide and a single transmembrane helix as characterized by structural and proteomic identification.
• Exhibits alternative splicing, producing multiple isoforms; the canonical sequence has a calculated molecular weight of approximately 14 kDa.
• Highly expressed in heart, placenta, and kidney, with intermediate levels in lung, skeletal muscle, and pancreas, suggesting tissue-specific roles in development and differentiation.
• Keywords associated: angiogenesis, apoptosis, cell adhesion, developmental protein, differentiation, and disulfide bond, reflecting its multifaceted roles in signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50-129 of human TWEAKR/Fn14/CD266, which lies within the extracellular domain; this region is well-exposed on the membrane surface, making the antibody suitable for detection of native receptor.
• For Western blot analysis, a band at approximately 14 kDa is expected under reducing conditions; however, potential glycosylation or dimerization may result in additional bands—validate in your specific sample system.
• Cross-reactivity with mouse allows application in mouse-derived samples; consider confirming target identity using knockout tissue or siRNA knockdown controls.
• For ELISA, the antibody can be employed as a capture or detection reagent; optimal pairing and dilution should be determined empirically using recombinant protein or cell lysates.
• As a recombinant monoclonal antibody, it offers high batch-to-batch consistency; always include appropriate positive and negative controls to ensure assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your diagnostic development by providing comprehensive IVD raw materials. This anti-TWEAKR/Fn14/CD266 antibody supports research into angiogenesis and apoptosis pathways. Beyond antibodies, we offer bulk ancillary reagents and sourcing for rare targets—ensuring a reliable supply chain from concept to clinic. Whether you need validated antibody pairs or custom monoclonal production, CamelBio delivers one-stop solutions tailored to diagnostic manufacturers.