Anti-TWEAKR/Fn14/CD266 Polyclonal Antibody for WB - Q9NP84

Core Product Specifications and Parameters

Biological Background: TWEAKR/Fn14/CD266 Function and Localization

• TWEAKR (TNFRSF12A, also known as Fn14/CD266) is the receptor for the cytokine TWEAK (TNFSF12), a member of the tumor necrosis factor superfamily.
• Upon ligand binding, TWEAKR weakly induces apoptosis in some cell types and promotes angiogenesis and endothelial cell proliferation, implicating it in tissue repair and inflammation.
• The protein may also modulate cellular adhesion to matrix proteins, suggesting a role in cell-matrix interactions and migration.
• TWEAKR is a single-pass type I membrane protein, located at the plasma membrane, and contains a cysteine-rich domain characteristic of TNF receptor family members.
• It is expressed highly in heart, placenta, and kidney, with intermediate levels in lung, skeletal muscle, and pancreas, indicating tissue-specific functions.
• Alternative splicing generates multiple transcript variants, potentially contributing to functional diversity.
• Key structural features include a signal peptide, a transmembrane helix, and conserved disulfide bonds, ensuring proper folding and receptor signaling.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 28-129), suggesting the antibody recognizes the ligand-binding region; confirm specificity via knockdown or knockout controls in WB experiments.
• For Western blot, use reducing SDS-PAGE; the predicted molecular weight is approximately 14 kDa, but consider that glycosylation may cause a slightly higher apparent mass.
• In ELISA, the antibody can be used as a capture or detection reagent; optimize coating conditions and antibody concentrations with a recombinant TWEAKR protein standard.
• When working with human samples, note the high expression in heart, placenta, and kidney; validate performance in lysates from these tissues or relevant cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, from validated antibody pairs to bulk ancillary reagents, supporting your TNFRSF12A/TWEAKR research needs. We supply this anti-TWEAKR polyclonal antibody for applications like WB and ELISA, and can assist in sourcing rare-target reagents or developing custom antibody sets for your diagnostic assays. Our B2B platform streamlines procurement, helping you move from concept to clinic with reliable, high-quality materials.