Anti-TUG/ASPSCR1 Rabbit Monoclonal Antibody for WB and ELISA - Q9BZE9

Core Product Specifications and Parameters

Biological Background: TUG/ASPSCR1 Function and Localization

• TUG/ASPSCR1 (Tether containing UBX domain for GLUT4) is a tethering protein that sequesters GLUT4 vesicles in the cytoplasm under basal conditions, thereby modulating insulin-stimulated glucose uptake.
• It is also known as alveolar soft part sarcoma chromosomal region candidate gene 1 protein (ASPSCR1), renal papillary cell carcinoma protein 17 (RCC17), and UBX domain-containing protein 9 (UBXD9/UBXN9).
• In addition to GLUT4 regulation, TUG/ASPSCR1 enhances the methylation of valosin-containing protein (VCP) catalyzed by VCPKMT.
• The protein localizes to the endomembrane system, specifically the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membrane, and is also detected in the cytoplasm and nucleus.
• TUG/ASPSCR1 is ubiquitously expressed, with highest levels in testis, heart, skeletal muscle, and pancreas.
• Post-translational modifications include acetylation and phosphorylation, although specific sites remain to be characterized.
• Chromosomal rearrangements involving the ASPSCR1 gene, such as the ASPSCR1-TFE3 fusion, are associated with alveolar soft part sarcoma and other malignancies, classifying it as a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 200-300) targets a conserved central region of human TUG/ASPSCR1; cross-reactivity with mouse is reported, and other species may be predicted based on sequence alignment.
• For Western Blot, consider using lysates from cell lines or tissues with known GLUT4 trafficking activity (e.g., muscle, fat) and validate with positive controls where possible.
• In ELISA, indirect or sandwich formats may be optimized by titrating the antibody and using recombinant or native antigen; inclusion of a negative control (immunogen peptide block) can confirm specificity.
• Since TUG/ASPSCR1 is a cytoplasmic and membrane-associated protein, gentle lysis buffers are recommended to preserve epitope integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, research laboratories, and institutes with high-quality IVD raw materials, including validated monoclonal antibodies like this anti-TUG/ASPSCR1 clone for glucose metabolism and cancer biomarker studies. We offer a full range of support, from antibody pairs and bulk ancillary reagents to custom sourcing of rare-target antibodies, helping you streamline assay development from concept to clinical application. Partner with CamelBio to leverage our comprehensive supply chain and technical expertise for your next diagnostic project.