Anti-TUFM Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P49411

Core Product Specifications and Parameters

Biological Background: TUFM Function and Localization

• TUFM encodes the mitochondrial elongation factor Tu (EF-TuMT), also known as P43, which is essential for mitochondrial protein biosynthesis.
• As a GTP hydrolase, EF-TuMT promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during mitochondrial translation, a process conserved across species.
• Beyond translation, TUFM plays a role in regulating autophagy and innate immunity by recruiting ATG5-ATG12 and NLRX1 at mitochondria, thereby serving as a checkpoint for the RIGI-MAVS pathway. Related references: PMID:28407488
• TUFM inhibits RLR-mediated type I interferon responses while promoting autophagy, linking mitochondrial protein synthesis to antiviral innate immune signaling. Related references: PMID:22749352
• Subcellularly, TUFM is localized to the mitochondrial matrix, consistent with its primary function in organellar translation.
• Key post-translational modifications include acetylation and phosphorylation, as identified in proteomics studies, which may regulate its activity or interactions.
• Mutations in TUFM are associated with primary mitochondrial disease, underscoring its clinical significance in mitochondrial dysfunction.
• TUFM is also implicated in host-virus interactions, as indicated by functional keyword annotations, though detailed mechanisms remain to be fully elucidated.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 186-455) of human TUFM; cross-reactivity with mouse and rat is expected due to high sequence conservation, but sequence alignment validation is recommended for other species.
• For WB, a band near 50 kDa is anticipated in mitochondrial-enriched lysates; consider using appropriate subcellular fractionation controls.
• In IHC-P and IF/ICC, a punctate cytoplasmic staining pattern typical of mitochondrial proteins should be observed; include mitochondrial marker co-staining for confirmation.
• The polyclonal nature of this antibody may provide robust signal for IP and ELISA applications; optimization of antibody concentration and incubation conditions is advised.
• As TUFM participates in autophagy regulation, treatments with autophagy inducers or inhibitors may modulate its expression or localization; consider this when designing functional assays.
• Antibody specificity is best validated using TUFM knockout or knockdown cell lines, or by peptide competition assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with end-to-end IVD raw material solutions, from concept to clinic. This anti-TUFM polyclonal antibody is part of our portfolio for mitochondrial and innate immunity research. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development and production.