Anti-TUBGCP3 Rabbit Polyclonal Antibody for WB, ELISA - Q96CW5

Core Product Specifications and Parameters

Biological Background: TUBGCP3 Function and Localization

• TUBGCP3 (Gamma-tubulin complex component 3) is also known as h104p, hGrip104, or hSpc98, and belongs to the tubulin gamma complex component family.
• It is a core component of the gamma-tubulin ring complex (γTuRC), which mediates microtubule nucleation—a fundamental process for cellular organization and division. Related references: PMID:38305685 | PMID:38609661 | PMID:39321809
• Through the γTuRC, TUBGCP3 facilitates the minus-end nucleation of α/β-tubulin heterodimers into microtubule protofilaments, which is essential for centrosome duplication and mitotic spindle formation. Related references: PMID:38305685 | PMID:38609661 | PMID:39321809
• The protein localizes to the cytoplasm, specifically at the microtubule organizing center (centrosome), where it exerts its nucleation function.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or complex assembly.
• The full-length protein has a calculated molecular mass of approximately 104 kDa.
• TUBGCP3 is ubiquitously expressed across human tissues, consistent with its role in fundamental microtubule processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-250) of human TUBGCP3; therefore, the antibody may be particularly useful for detecting epitopes within this domain—consider this for epitope mapping or competitive assays.
• For Western blot, expect a band around 104 kDa; validate the protein’s migration in your specific sample system, as post-translational modifications may alter observed mobility.
• This rabbit polyclonal antibody may recognize multiple isoforms or orthologs; verify specificity by knockdown/knockout controls or mass spectrometry if needed.
• For ELISA applications, the recombinant immunogen can serve as a positive control to establish assay conditions.
• The antibody is reported to cross-react with human, mouse, and rat; thus it is suitable for comparative studies across these species, but independent validation in the intended tissue or cell line is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for IVD raw-material sourcing, providing a comprehensive portfolio of validated antibodies, bulk ancillary reagents, and support services to streamline your diagnostic development. This anti-TUBGCP3 antibody supports research in centrosome biology, microtubule dynamics, and cell cycle regulation. Leverage our one-stop platform to access optimized monoclonal/polyclonal antibodies and rare-target raw materials that accelerate your project from discovery to clinical application.