Anti-TUBB8 Polyclonal Antibody for WB, IF/ICC, ELISA - Q3ZCM7

Core Product Specifications and Parameters

Biological Background: TUBB8 Function and Localization

• TUBB8 encodes the beta-8 chain of tubulin, which is a major constituent of microtubules. Microtubules are cylindrical polymers composed of alpha- and beta-tubulin heterodimers that assemble into linear protofilaments.
• TUBB8 plays a critical role in meiotic spindle assembly and is essential for proper chromosome segregation during oocyte maturation. Related references: PMID:26789871, PMID:34509376
• TUBB8 is predominantly expressed in oocytes at different stages of development, highlighting its specialized function in female meiosis.
• Subcellularly, TUBB8 localizes to the cytoplasm, cytoskeleton, and specifically the meiotic spindle, consistent with its role in microtubule dynamics.
• Like other beta-tubulins, TUBB8 binds GTP, and its GTPase activity is essential for microtubule dynamic instability; the alpha-subunit hydrolyzes GTP, while the beta-subunit is in the GTP-bound form at the growing microtubule plus end.
• TUBB8 is a phosphoprotein and contains isopeptide bonds, with potential post-translational modifications that regulate its function.
• Mutations in TUBB8 are associated with oocyte maturation defects and female infertility, underscoring its clinical significance in reproductive medicine.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody corresponds to amino acids 344-444 of human TUBB8. This region is within the C-terminal domain, which may influence specificity across beta-tubulin isoforms; cross-reactivity testing with other tubulins is recommended if used in non-oocyte systems.
• For western blotting (WB) of human samples, TUBB8 is predicted to run at an apparent molecular weight of ~50 kDa. Use appropriate positive controls such as human oocyte or ovarian tissue lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), TUBB8 staining is expected to localize to the meiotic spindle in oocytes; fixation and permeabilization conditions should be optimized to preserve microtubule structures.
• ELISA applications can be used for peptide-based quantification; ensure that the coating antigen matches the immunogen region.
• Since TUBB8 expression is highly restricted to oocytes, other cell lines may show low or no endogenous expression; forced expression models or in vitro differentiated germ cells may be considered for functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from assay concept to clinical application. This anti-TUBB8 polyclonal antibody serves as a reliable reagent for reproductive biology and infertility research, addressing the need for oocyte- and meiosis-specific markers. Beyond individual products, CamelBio offers validated antibody pairs for IVD kits, bulk quantities of ancillary reagents, and customized sourcing for rare targets, ensuring a seamless supply chain for your development pipeline.