Anti-TTR Rabbit Monoclonal Antibody for WB, ELISA - P02766

Core Product Specifications and Parameters

Biological Background: Transthyretin Function and Localization

• Transthyretin (TTR), also known as prealbumin or ATTR, is a thyroid hormone-binding protein encoded by the TTR gene (GeneID: 7276).
• It functions as a transporter of thyroxine (T4) from the bloodstream to the brain, playing a critical role in thyroid hormone homeostasis.
• TTR is primarily secreted and detected in serum and cerebrospinal fluid, with high expression in choroid plexus epithelial cells, retina pigment epithelium, and liver.
• Subcellular localization is both secreted and cytoplasmic, reflecting its synthesis and secretion pathway.
• Post-translational modifications include glycosylation, phosphorylation, sulfation, and gamma-carboxyglutamic acid modification.
• Mutations in TTR are associated with amyloidosis, including familial amyloid polyneuropathy and senile systemic amyloidosis, where misfolded TTR forms amyloid fibrils.
• The protein's molecular weight is approximately 16 kDa (UniProt: 15.9 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 68-147 of human TTR, suggesting that the antibody likely recognizes a linear epitope. This makes it suitable for Western blot under denaturing conditions.
• For Western blot, consider using reducing SDS-PAGE and a positive control such as human serum or liver lysate; a band at approximately 16 kDa is expected.
• For ELISA, validate performance using recombinant human TTR or serum samples; optimal dilution and conditions should be determined empirically.
• Since the antibody is rabbit monoclonal, it offers high specificity and consistency, but always include appropriate controls in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this high-quality anti-TTR antibody alongside a wide portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether your research focuses on thyroid hormone transport or amyloidosis, our team can assist in sourcing rare targets and scaling up production to meet your diagnostic development needs.