Anti-TTLL12 Polyclonal Antibody for WB and ELISA - Q14166

Core Product Specifications and Parameters

Biological Background: TTLL12 Function and Localization

• TTLL12 (Tubulin--tyrosine ligase-like protein 12) is an inactive member of the tubulin-tyrosine ligase (TTL) family, lacking catalytic activity for tubulin ligation.
• It negatively regulates tubulin post-translational modifications, specifically detyrosination of the C-terminus and polyglutamylation of glutamate residues, impacting microtubule dynamics and stability (PubMed:20162578, PubMed:23251473). Related references: PMID:20162578 PMID:23251473
• TTLL12 indirectly promotes histone H4 trimethylation at lysine 20 (H4K20me3), a chromatin modification associated with transcriptional repression and genome stability (PubMed:23251473). Related references: PMID:23251473
• By modulating tubulin and histone modifications, TTLL12 plays a role in mitosis and chromosome number stability, helping to prevent aneuploidy (PubMed:20162578, PubMed:23251473). Related references: PMID:20162578 PMID:23251473
• In the antiviral innate immune response, TTLL12 acts as a negative regulator of the RIG-I pathway by inhibiting the interaction between MAVS and TBK1/IKBKE, thereby dampening interferon production during RNA virus infection (PubMed:28011935). Related references: PMID:28011935
• Subcellularly, TTLL12 localizes to the cytoplasm, midbody, microtubule organizing center (centrosome), spindle, and nucleus, consistent with its roles in cytoskeleton and chromatin regulation.
• Tissue expression is prominent in the basal layer of prostate and endothelial cells, with upregulated levels observed in prostatic intraepithelial neoplasia and metastatic lesions, suggesting involvement in cancer progression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 485-644) of human TTLL12, a domain that may contain unique epitopes not conserved in other TTL family members, enhancing specificity.
• For Western blot analysis, consider using whole-cell lysates from human prostate or endothelial cell lines (e.g., PC-3, HUVEC) where TTLL12 is expressed. The predicted molecular weight is approximately 74 kDa; however, validate the observed band size in your sample system.
• Due to nuclear and cytoplasmic localization, subcellular fractionation may help enrich TTLL12 signal in WB.
• For ELISA applications, the polyclonal nature provides robust detection sensitivity; optimize coating and detection conditions using recombinant TTLL12 protein as standard.
• Cross-reactivity with mouse and rat allows for translational studies in these species; confirm epitope conservation and adjust antibody dilutions accordingly.
• As TTLL12 is involved in mitosis and antiviral pathways, consider synchronizing cells or stimulating with viral RNA mimics to study dynamic expression changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare-target procurement. This anti-TTLL12 antibody is ideal for research on tubulin biology, chromatin modification, cancer, and innate immunity. Leverage CamelBio's one-stop supply chain to accelerate your diagnostic assay development from concept to clinic.