Anti-TTK Polyclonal Antibody for WB, IF/ICC, ELISA - P33981

Core Product Specifications and Parameters

Biological Background: TTK Function and Localization

• Dual specificity protein kinase TTK (also known as MPS1) is a serine/threonine and tyrosine kinase that plays a critical role in the mitotic spindle assembly checkpoint, delaying anaphase until all chromosomes are correctly bioriented, and in correcting erroneous kinetochore-microtubule attachments.
  Related references: PMID:18243099, PMID:28441529, PMID:29162720
• TTK phosphorylates MAD1L1 to promote the mitotic spindle assembly checkpoint.
  Related references: PMID:18243099, PMID:29162720
• It phosphorylates CDCA8/Borealin, enhancing Aurora B kinase (AURKB) activity at the kinetochore.
  Related references: PMID:18243099
• Phosphorylation of SKA3 at Ser-34 by TTK triggers dissociation of the SKA complex from microtubules, destabilizing microtubule-kinetochore attachments.
  Related references: PMID:28441529
• TTK phosphorylates KNL1, KNTC1, and also autophosphorylates, contributing to checkpoint signaling.
  Related references: PMID:28441529
• It phosphorylates MCRS1, enhancing recruitment of KIF2A to the minus ends of spindle microtubules, which promotes chromosome alignment.
  Related references: PMID:30785839
• The protein is present in rapidly proliferating cell lines, consistent with its mitotic functions.
• TTK contains an ATP-binding kinase domain, undergoes acetylation and alternative splicing, and is catalogued as a reference proteome phosphoprotein.

Experimental Guidance and Technical Tips

• For Western blotting, the immunogen targets the C-terminal region (aa 536-789) of human TTK. The predicted molecular weight is ~97 kDa; confirm band migration in your specific lysates and conditions.
• In immunofluorescence/ICC, TTK localizes to kinetochores during mitosis. Use appropriate permeabilization and fixation protocols for optimal kinetochore staining. Validate in mitotic cell populations (e.g., nocodazole-treated cells).
• For ELISA applications, titrate the antibody as both capture and detection reagent to determine suitable working concentrations. Include positive and negative controls.
• The antibody cross-reacts with human, mouse, and rat TTK, but sequence homology in the immunogen region suggests possible reactivity with other species; validation in the target species is recommended.
• Consider using an appropriate loading control and phospho-specific antibodies for functional studies of TTK kinase activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive sourcing platform for IVD raw materials, including TTK antibodies and related cell cycle regulators. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare and custom targets to support your assay development from concept to clinic. Our team can help you secure the right raw materials for robust and reproducible diagnostic kits.