Anti-TTF2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UNY4

Core Product Specifications and Parameters

Biological Background: TTF2 Function and Localization

• TTF2 (Transcription Termination Factor 2), also known as Lodestar homolog, HuF2, or ZGRF6, is a member of the SNF2/RAD54 helicase family.
• Functions as a dsDNA-dependent ATPase that couples ATP hydrolysis with the removal of RNA polymerase II from DNA, thereby mediating transcription termination.
• May contribute to global transcription repression during mitosis, ensuring proper chromosome segregation.
• Has been implicated in pre-mRNA splicing through its association with the spliceosome, suggesting a dual role in transcription and RNA processing.
• Localizes to both the cytoplasm and nucleus, reflecting its involvement in nuclear transcription and potential cytoplasmic functions.
• Contains conserved ATP-binding/helicase domains, a zinc-finger motif, and is regulated by phosphorylation and ubiquitination (isopeptide bond).
• UniProt keywords highlight its roles in ATP-binding, DNA-binding, helicase activity, hydrolysis, nucleotide-binding, transcription, transcription regulation, transcription termination, mRNA processing, mRNA splicing, and its status as a phosphoprotein subject to Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–270) of human TTF2, covering part of the conserved ATPase/helicase domain; the resulting polyclonal antibody may recognize multiple epitopes and detect various splice variants.
• In WB, the predicted molecular weight is ~130 kDa; a single major band or multiple bands may appear depending on cell line and post-translational modifications; validate using human cell lysates.
• For IF/ICC, nuclear and cytoplasmic staining patterns are expected; optimize fixation and permeabilization conditions for your cell type.
• ELISA can be performed with recombinant TTF2 protein or cell extracts; polyclonal antibodies often provide high sensitivity due to multi-epitope recognition.
• Cross-reactivity is validated for human; for other species, check sequence homology of the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-TTF2 polyclonal antibody as part of our comprehensive IVD raw material portfolio, supporting transcription termination and gene regulation research. As your trusted one-stop partner, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rapid sourcing of rare-target raw materials—streamlining your journey from concept to clinic.