Anti-TTF1 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q15361

Core Product Specifications and Parameters

Biological Background: TTF1 Function and Localization

• TTF1 is a multifunctional nucleolar protein that acts as a transcription termination factor for RNA polymerase I (Pol I) on ribosomal DNA (rDNA).
• It mediates replication fork arrest at the rDNA termination site, thereby contributing to DNA replication control.
• TTF1 plays a dual role in rDNA regulation, being involved in both transcriptional activation and silencing of ribosomal genes.
• Interaction with BAZ2A/TIP5 (part of the NoRC complex) can recover TTF1’s DNA-binding activity, linking it to heterochromatin formation and gene silencing.
• The protein localizes predominantly to the nucleus, with enrichment in the nucleolus and presence in the nucleoplasm.
• TTF1 is subject to post-translational modifications including phosphorylation and ubiquitin-like conjugation, and contains DNA-binding domains and repeat regions.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 806–905) of human TTF1; consider that the antibody may recognize epitopes in this region and may detect full-length TTF1 as well as any C-terminal isoforms.
• For immunofluorescence (IF/ICC), optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) to ensure adequate nuclear and nucleolar staining.
• In ELISA, validate the antibody’s performance with recombinant TTF1 protein or cell lysates from appropriate human cell lines to confirm specificity and sensitivity.
• As a polyclonal antibody, it may offer higher sensitivity due to recognition of multiple epitopes; however, lot-to-lot variability should be assessed for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to provide reliable IVD raw materials. Our one-stop sourcing includes validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and rare target sourcing for transcription and nucleolar biology. Leverage CamelBio’s supply chain expertise to accelerate your assay development from concept to clinic.