Anti-TTF-1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P43699

Core Product Specifications and Parameters

Biological Background: TTF-1 (NKX2-1) Function and Localization

• Identified as homeobox protein Nkx-2.1, also known as thyroid transcription factor 1 (TTF-1), thyroid-specific enhancer-binding protein (T/EBP), and NKX2.1; encoded by the NKX2-1 gene in humans.
• Functions as a transcription factor that binds and activates promoters of thyroid-specific genes (e.g., thyroglobulin, thyroperoxidase, thyrotropin receptor), crucial for maintaining thyroid differentiation phenotype.
• Plays an important role in lung development and surfactant homeostasis; forms a regulatory loop with GRHL2 to coordinate lung epithelial cell morphogenesis and differentiation.
• Activates transcription of GNRHR and enhances circadian oscillation of its gene expression; represses transcription of the circadian transcriptional repressor NR1D1.
• Localizes to the nucleus.
• Exhibits tissue-specific expression in thyroid and lung.
• Contains a homeobox DNA-binding domain, characteristic of the NK-2 family; subject to phosphorylation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (amino acids 1-100) of human NKX2-1; consider verifying epitope accessibility in native protein conformations or fixed samples.
• For Western blot, the predicted molecular weight is approximately 39 kDa; however, phosphorylation or other post-translational modifications may cause band shifts.
• Suitable for various applications including WB, IF-P, IHC-P, and ELISA; cross-reacts with human, mouse, and rat, indicating high sequence conservation.
• For immunohistochemistry (IHC-P) or immunofluorescence (IF-P), optimize antigen retrieval conditions for your specific tissue and fixation method.
• Validate the antibody in your relevant sample system, as performance may vary based on expression levels and sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio connects diagnostic developers with reliable antibodies like this anti-TTF-1 monoclonal antibody, supporting thyroid and lung research applications. We offer one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials—streamlining your journey from concept to clinic.