Anti-TTC5 Polyclonal Antibody for WB, ELISA - Q8N0Z6

Core Product Specifications and Parameters

Biological Background: TTC5 Function and Localization

• TTC5, also known as Strap (Stress-responsive activator of p300), is a tetratricopeptide repeat (TPR) protein that functions as a cofactor in diverse cellular processes including actin dynamics, autophagy, chromatin regulation, and DNA repair. Related references: PMID:18451878 PMID:31727855
• Under non-stress conditions, cytoplasmic TTC5 binds JMY, inhibiting JMY’s actin nucleation and Arp2/3 activation, and also suppresses autophagy by blocking JMY’s interaction with MAP1LC3B.
• In response to DNA damage, TTC5 is phosphorylated by ATM and CHEK2 and accumulates in the nucleus, where it facilitates assembly of a p53 coactivator complex with JMY and p300, enhancing p53-dependent transcription and apoptosis.
• TTC5 participates in tubulin autoregulation by associating with ribosomes and nascent tubulin polypeptides, triggering tubulin mRNA degradation when soluble tubulin is in excess. Related references: PMID:31727855
• Following heat shock, TTC5 forms a chromatin-associated complex with HSF1 and p300 to stimulate heat-shock-responsive transcription, thereby promoting cell survival. Related references: PMID:18451878
• Subcellularly, TTC5 localizes to the nucleus, cytoplasm, cytoplasmic vesicles, and mitochondrial matrix, where it interacts with ATP synthase subunit beta to modulate ATP production and apoptosis.
• UniProt keywords include DNA damage, DNA repair, phosphoprotein, TPR repeat, and intellectual disability, reflecting its roles in genome stability and disease association.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-300 of human TTC5, covering N-terminal TPR motifs; ensure the antibody is validated in the relevant species and sample type before use.
• For Western blot, a band at approximately 49 kDa is expected; optimize lysate preparation and primary antibody dilution for best signal specificity.
• In ELISA, consider direct or sandwich formats and titrate the antibody to determine the optimal working concentration for your assay system.
• Cross-reactivity with mouse and rat TTC5 is indicated; sequence alignment of the immunogen region may aid in predicting reactivity with additional orthologs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents, streamlining your procurement for chromatin biology and DNA damage research. With this TTC5 polyclonal antibody, we support your assay development in Western blot and ELISA applications across human, mouse, and rat models. Contact us to explore custom sourcing for rare targets and technical services from concept to clinical application.