Anti-TTC3 Rabbit Polyclonal Antibody for WB, ELISA - P53804

Core Product Specifications and Parameters

Biological Background: TTC3 Function and Localization

• TTC3 is an E3 ubiquitin-protein ligase that specifically catalyzes the formation of Lys-48-linked polyubiquitin chains, targeting substrates for proteasomal degradation. Related references: PMID:20059950, PMID:30696809
• It mediates ubiquitination and degradation of phosphorylated Akt (AKT1, AKT2, AKT3) in the nucleus, serving as a terminal regulator of Akt signaling; its own phosphorylation by Akt is a prerequisite for ligase activity. Related references: PMID:20059950
• Positively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation by ubiquitinating and degrading SMURF2. Related references: PMID:30696809
• Regulates neuronal differentiation by modulating actin remodeling and Golgi organization via the RHOA-CIT-ROCK signaling cascade. Related references: PMID:17488780, PMID:24695496
• Inhibits cell proliferation. Related references: PMID:30203323
• Localizes to the nucleus, cytoplasm, and Golgi apparatus.
• Ubiquitously expressed across all examined tissues.
• Features TPR repeats and a RING finger domain; classified under transferases and the ubiquitin conjugation pathway, with alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1400–1700, a region without predicted transmembrane domains, suitable for detecting both full-length and truncated isoforms in western blotting.
• For WB, the predicted molecular weight is ~230 kDa; consider using a low‑percentage SDS‑PAGE gel and wet transfer conditions optimized for high‑molecular‑weight proteins.
• TTC3 expression is ubiquitous but may vary between cell lines; include appropriate positive controls (e.g., HeLa, HEK293) and validate in your model system.
• When probing ubiquitination‑dependent degradation pathways, treatment with proteasome inhibitors (e.g., MG132) may stabilize the target and enhance detection.
• For ELISA, the polyclonal nature may provide robust signal but always titrate the antibody and confirm specificity with recombinant antigen or peptide blocking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories with validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for IVD assay development. This anti-TTC3 antibody can be integrated into ubiquitination and cell signaling research workflows, backed by our expertise in sourcing rare‑target raw materials. From concept to clinic, we offer dependable supply solutions tailored to your project needs.