Anti-TTC11/FIS1 Polyclonal Antibody for WB, IP, IF/ICC, ELISA - Q9Y3D6

Core Product Specifications and Parameters

Biological Background: TTC11/FIS1 Function and Localization

• Also known as FIS1 homolog (hFis1), Tetratricopeptide repeat protein 11 (TPR repeat protein 11), TTC11, and CGI-135.
• Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Related references: PMID:12783892 PMID:12861026 PMID:14996942
• Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Related references: PMID:12861026 PMID:16118244 PMID:23283981
• May not be essential for the assembly of functional fission complexes and the subsequent membrane scission event. Related references: PMID:23530241 PMID:24196833
• Also mediates peroxisomal fission. Related references: PMID:16107562
• Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Related references: PMID:12783892
• Localizes to the mitochondrial outer membrane and peroxisomal membrane.
• Contains TPR repeat domains, a transmembrane helix, and undergoes post‑translational modifications including acetylation, phosphorylation, and ubiquitylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (aa 1‑122), which likely represents the cytosolic domain; the antibody is expected to recognize the endogenous full‑length protein.
• For Western blotting, use standard SDS‑PAGE and transfer conditions suitable for a 17 kDa membrane‑associated protein.
• For immunoprecipitation, consider cross‑linking protocols if the target is part of large membrane‑associated complexes.
• For immunofluorescence/ICC, optimize permeabilization and fixation to preserve mitochondrial morphology.
• Validate the antibody in the relevant species (human, mouse, rat) and model system to confirm specificity and performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw‑material supply solutions, supporting diagnostic manufacturers from concept to clinic. This anti‑TTC11/FIS1 polyclonal antibody is part of our extensive catalog for mitochondrial and apoptosis research, ideal for assay development. We also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to accelerate your project timelines.