Anti-TTBK2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q6IQ55

Core Product Specifications and Parameters

Biological Background: TTBK2 Function and Localization

• TTBK2 (tau-tubulin kinase 2) is a serine/threonine protein kinase encoded by the TTBK2 gene; alternative splicing generates multiple isoforms.
• Acts as a key regulator of ciliogenesis: binds to the distal end of the basal body and promotes removal of CCP110, capping the mother centriole, allowing recruitment of IFT proteins to build the ciliary axoneme (PubMed:23141541). Additionally, TTBK2 phosphorylates MPHOSPH9, targeting it for degradation, which facilitates removal of the CP110-CEP97 complex from mother centrioles (PubMed:30375385). Related references: PMID:23141541 PMID:30375385
• Phosphorylates tau protein on serines in vitro, linking TTBK2 to tau pathology (PubMed:23141541). Related references: PMID:23141541
• Localizes to multiple subcellular compartments, including the cilium basal body, centrosome, centriole, cytosol, and nucleus.
• Classified as a phosphoprotein and is associated with spinocerebellar ataxia type 11 (SCA11), a neurodegenerative disorder.
• Keywords include ATP-binding, nucleotide-binding, kinase, transferase, and cilium biogenesis/degradation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1185-1244 near the C-terminus; the antibody may detect full-length TTBK2 and certain isoforms.
• For Western blotting, the predicted molecular weight is 137 kDa; consider that post-translational modifications (e.g., phosphorylation) may shift migration.
• When using for immunohistochemistry (IHC-P), optimize antigen retrieval conditions for your specific tissue and fixation.
• ELISA applications may be employed with recombinant or enriched endogenous protein samples; validate assay conditions.
• Cross-reactivity with rat is reported; validate in the relevant species prior to experimental use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with reliable access to a broad portfolio of IVD raw materials, including validated antibodies like this anti-TTBK2 polyclonal antibody. Whether you need optimized antibody pairs, bulk ancillary reagents, or sourcing support for rare targets, CamelBio serves as a streamlined procurement bridge from concept to clinic. Our expertise in molecular biology and IVD development ensures that you receive consistent, high-quality reagents to accelerate your neurodegeneration or ciliopathy research programs.