Anti-TSSK4 Rabbit Polyclonal Antibody for WB and ELISA - Q6SA08

Core Product Specifications and Parameters

Biological Background: TSSK4 Function and Localization

• TSSK4 is a testis-specific serine/threonine kinase (alias STK22E) involved in male germ cell development and mature sperm function.
• It phosphorylates CREB1 on Ser-133 and stimulates the Cre/Creb signaling pathway in vitro (PubMed:15964553). Related references: PMID:15964553
• It phosphorylates CREM on Ser-116 and ODF2 on Ser-95, linking it to sperm tail development (By similarity).
• Localizes to the acrosome (secretory vesicle) and flagellum of sperm, consistent with roles in acrosome reaction and motility.
• Belongs to the CAMK Ser/Thr protein kinase family; binds ATP, magnesium, and is regulated by phosphorylation and ubiquitination.
• Expressed exclusively in the testis, as a developmental protein critical for spermatogenesis.
• Alternative splicing produces isoforms (e.g., TSSK4 isoform 1).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 79-328 of human TSSK4, covering most of the kinase domain; this region may recognize isoforms and is suitable for WB detection.
• Predicted molecular weight is ~37 kDa; for best results, use testis tissue or cell lines that express TSSK4, as it is testis-specific.
• Cross-reactivity with mouse is provided; validate in relevant mouse testis samples if needed.
• Since TSSK4 is a kinase, consider phosphatase inhibitors during sample preparation to preserve phosphorylation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a reliable supply of high-quality antibodies for IVD assay development, including polyclonal antibodies like anti-TSSK4 for reproductive biology research. Our portfolio covers validated reagents for kinase signaling, spermatogenesis, and related diagnostic targets. Partner with us for comprehensive sourcing from concept to clinical application.