Anti-TSSK3 Polyclonal Antibody for WB, ELISA - Q96PN8

Core Product Specifications and Parameters

Biological Background: TSSK3 Function and Localization

• TSSK3 is a testis-specific serine/threonine protein kinase, also known as STK22C, encoded by the TSSK3 gene (GeneID: 81629).
• It is essential for spermatid development and male fertility.
• TSSK3 contains an ATP-binding domain and requires magnesium as a cofactor for its kinase activity.
• It localizes to the flagellum of sperm cells, consistent with a role in sperm motility and fertilization.
• TSSK3 belongs to the TSSK family of testis-specific serine/threonine kinases involved in spermatogenesis and cellular differentiation.
• Post-translational modifications include phosphorylation, and it has been identified in proteomics screenings.
• Its molecular weight is approximately 30 kDa (UniProt: 30102 Da), matching the observed protein weight.

Experimental Guidance and Technical Tips

• For Western blotting, use whole-cell lysates from testis tissues or relevant cell lines; include phosphatase inhibitors to maintain the phosphorylation state of TSSK3.
• In ELISA, consider pairing with a capture antibody for sandwich format and validate the assay in relevant biological matrices such as seminal plasma or testicular extracts.
• The immunogen spans the entire kinase domain (aa 1-268), so the antibody may detect both phosphorylated and unphosphorylated forms; optimize blocking conditions with non-fat milk or BSA.
• The antibody shows cross-reactivity with mouse and rat, enabling comparative studies across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research institutes. Our supply network supports rare targets such as TSSK3, facilitating reproductive health diagnostic and research assay development. With end-to-end technical consulting and procurement services, CamelBio accelerates your journey from concept to clinic.