Anti-TSPY3 Rabbit Polyclonal Antibody for WB and ELISA - P0CV98

Core Product Specifications and Parameters

Biological Background: TSPY3 Function and Localization

• TSPY3 (Testis-specific Y-encoded protein 3) is a member of the TSPY gene family located on the Y chromosome, sharing homology with other TSPY proteins like TSPY1 and TSPY10; it is also known as cancer/testis antigen 78 (CT78).
• It is predicted to play a role in sperm differentiation and proliferation, as indicated by its testis-specific expression and association with spermatogenesis-related keywords.
• Subcellularly, TSPY3 localizes to both the cytoplasm and nucleus, suggesting potential shuttling or multiple functional compartments during germ cell development.
• The protein is implicated in gonadal differentiation and developmental processes, consistent with its classification as a developmental protein.
• Molecular weight is approximately 35 kDa, as provided by both UniProt (35,101 Da) and the antibody supplier, consistent with the predicted size of the full-length protein.
• TSPY3 is part of a cluster of TSPY genes, which are often involved in cell cycle regulation and spermatogonial proliferation; however, specific functional validation studies may be limited.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length TSPY3 protein (amino acids 1–308), which is expected to recognize the native protein. This design may support detection in both denaturing (WB) and native (ELISA) conditions.
• For Western blotting, a band at approximately 35 kDa is anticipated in human testis or TSPY3-expressing cell lines. Optimize sample extraction and loading amounts as needed.
• Cross-reactivity with rat is reported; use caution when interpreting results in rodent models, and validate specificity in the relevant species.
• Consider testing the antibody in ELISA with recombinant TSPY3 protein as a standard for quantitative assays.
• Since TSPY3 may localize to both cytoplasm and nucleus, subcellular fractionation may enhance detection sensitivity in Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, including validated antibodies like this anti-TSPY3 polyclonal antibody. In addition to this product, we supply antibody pairs, bulk ancillary reagents, and can assist in sourcing rare-target raw materials for reproductive biology and cancer research applications. Contact us to streamline your supply chain from concept to clinic.