Anti-TSPAN8 Rabbit Polyclonal Antibody for WB, ELISA - P19075

Core Product Specifications and Parameters

Biological Background: TSPAN8 Function and Localization

• TSPAN8 (CO-029, TM4SF3) is a tetraspanin family protein that acts as a structural component of tetraspanin-enriched microdomains (TERMs), mediating receptor clustering, signal transduction, cell migration, and protein trafficking. Related references: PMID:27180357, PMID:36078095, PMID:25761241
• It promotes ADAM17-mediated TNF processing by recruiting ADAM17 to TEMs. Related references: PMID:36078095
• TSPAN8 forms a complex with RICTOR and integrin alpha3 to activate mTORC2 and AKT1 phosphorylation, driving cell migration. Related references: PMID:25761241
• It reduces high glucose-induced apoptosis and autophagy by forming a complex with mTOR and RICTOR. Related references: PMID:35904232
• TSPAN8 maintains intestinal epithelial barrier integrity and regulates inflammation by redirecting IFNGR1 endocytosis from clathrin-dependent to lipid raft-dependent routes, limiting STAT1 activation. Related references: PMID:37204469
• It modulates the endothelin axis by binding to endothelin-converting enzyme ECE1 and regulating endothelin-1 maturation. Related references: PMID:37835445
• Subcellular localization is predominantly at the cell membrane.
• TSPAN8 is highly expressed in gastric, colon, rectal, and pancreatic carcinomas.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 110–200 of human TSPAN8, which lies within a loop region likely extracellular; thus, the antibody may recognize epitopes sensitive to reduction or membrane disruption.
• For Western blotting, test under both reducing and non-reducing conditions to account for potential disulfide bond-dependent epitopes, as TSPAN8 contains conserved cysteine residues typical of tetraspanins.
• In ELISA, consider using detergent-solubilized membrane fractions or recombinant extracellular domains to preserve native conformation, as the target is an integral membrane protein.
• Cross-reactivity is reported with mouse and rat; thus, the antibody can be considered for rodent model studies, but always validate specificity in the relevant sample system.
• When working with tetraspanin-enriched microdomains, include proper controls (e.g., lipid raft markers) to confirm membrane compartment-specific detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This TSPAN8 antibody supports research into tetraspanin biology, cancer diagnostics, and intestinal barrier function. Beyond antibodies, we offer validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to streamline your assay development pipeline.