Anti-TSPAN8 Rabbit Polyclonal Antibody for WB, ELISA - P19075

Core Product Specifications and Parameters

Biological Background: TSPAN8 Function and Localization

• TSPAN8 is a structural component of tetraspanin-enriched microdomains (TERMs), which serve as platforms for receptor clustering and signaling. Related references: PMID:27180357 PMID:36078095
• Participates in diverse biological functions including cell signal transduction, migration, and protein trafficking. Related references: PMID:25761241
• Promotes ADAM17-mediated TNF processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs). Related references: PMID:36078095
• Forms a complex with RICTOR and integrin alpha3/ITGA3 to mediate mTORC2 activation and AKT1 phosphorylation leading to cell migration. Related references: PMID:25761241
• Reduces apoptosis and autophagy induced by high glucose levels through forming a complex with mTOR and RICTOR. Related references: PMID:35904232
• Contributes to the maintenance of intestinal epithelial barrier and plays a role in the regulation of intestine inflammation by switching interferon gamma receptor 1/IFNGR1 from clathrin-dependent to lipid raft-dependent endocytosis route to limit STAT1 activation magnitude and duration. Related references: PMID:37204469
• Acts as a modulator of the endothelin axis by associating with endothelin converting enzyme ECE1 and regulating its activity of conversion of the endothelin-1 precursor to endothelin. Related references: PMID:37835445
• Subcellularly localized to the cell membrane; tissue specificity includes gastric, colon, rectal, and pancreatic carcinomas. Keywords: glycoprotein, transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 110-200 of human TSPAN8. This region may be conserved across species, potentially contributing to the reported cross-reactivity with mouse and rat.
• For Western blot (WB), consider using cell lines known to express TSPAN8, such as gastric, colon, rectal, or pancreatic carcinoma cells. Confirm expression levels under relevant experimental conditions.
• For ELISA, validate with recombinant TSPAN8 protein or tissue/cell lysates. Optimize coating and detection conditions based on the specific assay format.
• As a polyclonal antibody, batch-to-batch variability is possible; consider including appropriate controls and, if needed, purify the antibody for specific applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers and researchers from concept to clinic. This TSPAN8 antibody, targeting tetraspanin-8—a protein involved in cell signaling and tumor progression—exemplifies our portfolio of high-quality, applications-validated antibodies. Beyond individual reagents, we supply validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development and manufacturing workflows.