Anti-TSPAN7 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P41732

Core Product Specifications and Parameters

Biological Background: TSPAN7 Function and Localization

• Tetraspanin-7 is a member of the tetraspanin superfamily, also known as the transmembrane 4 superfamily, and is encoded by the TSPAN7 gene. Alternative names include cell surface glycoprotein A15, T-cell acute lymphoblastic leukemia-associated antigen 1 (TALLA-1), and membrane component chromosome X surface marker 1 (MXS1).
• The protein is predicted to function in the regulation of cell proliferation and cell motility.
• Tetraspanin-7 is localized to the plasma membrane, consistent with its four-transmembrane domain structure.
• While originally associated with T-cells, TSPAN7 expression is not solely restricted to T-cells; it has been detected in acute myelocytic leukemia cells from some patients.
• The protein undergoes glycosylation and features key structural motifs of tetraspanins, including conserved transmembrane helices and characteristic extracellular loops.
• TSPAN7 is implicated in host-virus interactions and has been linked to disease variants associated with intellectual disability, highlighting its importance in both physiological and pathological processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region encompassing amino acids 140-240, which spans part of the large extracellular loop of TSPAN7. This region is likely exposed on the cell surface and may be accessible for antibody binding in native conditions.
• For Western blotting, the predicted molecular weight is approximately 28kDa; however, glycosylation may cause migration at a slightly higher apparent molecular weight. Verify using appropriate positive controls.
• This polyclonal antibody has been validated in WB, IF-P, and ELISA applications. Consider optimizing antibody dilutions and experimental conditions specific to your sample type.
• Cross-reactivity with mouse and rat TSPAN7 has been reported, making this antibody suitable for studies in these species, but confirm reactivity in the relevant tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials for targets like TSPAN7, which is relevant to cell proliferation, motility, and disease research. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to streamline your assay development. Our one-stop sourcing model also provides technical services and consulting, supporting your project from concept to clinic.