Anti-TSPAN4/NAG-2 Rabbit Polyclonal Antibody for WB, ELISA - O14817

Core Product Specifications and Parameters

Biological Background: TSPAN4/NAG-2 Function and Localization

• Tetraspanin-4 (TSPAN4), also known as NAG-2 and TM4SF7, is a member of the tetraspanin superfamily, characterized by four transmembrane domains.
• It acts as a structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), facilitating receptor clustering and signaling.
• TSPAN4 plays an essential role in migrasome formation and migration on retracting fibers at the rear of migrating cells.
  Related references: PMID:31371828
• It functions as a membrane curvature sensor and stabilizes the migrasome structure during late-stage biogenesis.
  Related references: PMID:36252000, PMID:36823145
• TSPAN4 may also regulate the histamine H4 receptor (HRH4) without affecting histamine binding or signaling.
  Related references: PMID:34439793
• Subcellularly, TSPAN4 is localized to the cell membrane.
• It is expressed in multiple tissues but is absent in brain, lymphoid cells, and platelets.
• Post-translationally, TSPAN4 is a glycoprotein with a calculated molecular weight of approximately 26 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 of human TSPAN4, a region within the large extracellular loop. This design may favor detection of the target in both native and denaturing conditions.
• For Western blotting, consider optimizing antibody concentration and using appropriate loading controls. The predicted molecular weight is ~26 kDa; however, post-translational modifications may affect migration.
• In ELISA applications, direct or indirect formats can be employed; titration is recommended to determine optimal working dilutions.
• Cross-reactivity with mouse and rat TSPAN4 has been noted; however, validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing comprehensive IVD raw-material solutions, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. With a focus on membrane protein targets like TSPAN4, CamelBio can assist diagnostic manufacturers and researchers in securing rare and high-quality raw materials for their assay development pipelines. Whether you require customized sourcing or technical consultation, CamelBio bridges the gap from concept to clinic.