Anti-TSPAN33 Polyclonal Antibody for WB, ELISA - Q86UF1

Core Product Specifications and Parameters

Biological Background: TSPAN33 Function and Localization

• TSPAN33 is a member of the tetraspanin family and belongs to the TspanC8 subgroup, which comprises six members that interact with the transmembrane metalloprotease ADAM10. This interaction is essential for ADAM10 exit from the endoplasmic reticulum, its enzymatic maturation, trafficking to the cell surface, and determination of substrate specificity. Distinct TspanC8/ADAM10 complexes exhibit different substrate preferences. Related references: PMID:26686862, PMID:30463011, PMID:37516108

• Plays a critical role in normal erythropoiesis and the differentiation of erythroid progenitors (By similarity).

• Negatively regulates ligand-induced Notch activity, likely by modulating ADAM10 function. Related references: PMID:26686862

• Mediates the docking of ADAM10 to the zonula adherens by simultaneously interacting with ADAM10 and, in a PDZD11-dependent manner, with the adherens junction protein PLEKHA7. Related references: PMID:30463011

• Predominantly expressed in erythroblasts, consistent with its role in red blood cell development.

• Localizes to the cell membrane, cell junctions (adherens junctions), and cytoplasm.

• Post-translational modifications include disulfide bond formation and glycosylation (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminus (amino acids 200 to end) of human TSPAN33. For WB, expect a band at approximately 32 kDa. Validate detection in appropriate sample systems, such as erythroblast cell lines.

• This polyclonal antibody is suitable for WB and ELISA. Consider using blocking peptides to confirm specificity in your assays.

• Cross-reactivity is reported for human, mouse, and rat samples. Verify performance in your specific model system and optimize dilution factors empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-TSPAN33 polyclonal antibody supports research in erythropoiesis and ADAM10-mediated signaling pathways. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing for rare target raw materials to streamline your development from concept to clinic.